CDK‐ and Cdc45‐dependent priming of the MCM complex on chromatin during S‐phase in Xenopus egg extracts: possible activation of MCM helicase by association with Cdc45

Background: MCM and Cdc45 are required for the initiation and elongation stages of eukaryotic DNA replication. Recent studies show that a purified Mcm4/6/7 complex has DNA helicase activity. However, the biochemical function of the MCM complex and Cdc45 bound to chromatin has not been elucidated.

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