Molecular Characterization of the Rat Insulin Enhancer-binding Complex 3b2

Cell-specific expression of the rat insulin II gene is in part mediated through an element located in the 5′-flanking region. The element, termed RIPE3b (−126 to −101), confers β-cell-specific expression in conjunction with an adjacent element RIPE3a (−110 to −86). Here we report the characterization of one of the RIPE3b-binding complexes, 3b2. UV cross-linking analysis demonstrated that it is composed of at least three polypeptides: p58, p62, and p110. Furthermore, a cDNA was isolated via expression screening for binding to RIPE3b. Sequence analysis reveals that the encoded protein, designated Rip-1, possessed putative helicase motifs and a potential transcription activation domain. Overexpression of Rip-1 in cells greatly enhances the 3b2 binding complex, suggesting that Rip-1 is involved in the binding of 3b2.

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