Intracellular click reaction with a fluorescent chemical Ca2+ indicator to prolong its cytosolic retention.

The powerful strategy of "intracellular click reaction" was used to retain a chemical Ca(2+) indicator in the cytosol. Specifically, a novel clickable Ca(2+) indicator "N3-fura-2 AM" was coupled with dibenzylcyclooctyl-modified biomacromolecules via copper-free click reaction in living cells and Ca(2+) oscillation was observed for an extended period of time.

[1]  Takuya Terai,et al.  Small-molecule fluorophores and fluorescent probes for bioimaging , 2013, Pflügers Archiv - European Journal of Physiology.

[2]  Sung Bae Kim,et al.  Advances in fluorescence and bioluminescence imaging. , 2013, Analytical chemistry.

[3]  C. Bertozzi,et al.  d-Amino Acid Chemical Reporters Reveal Peptidoglycan Dynamics of an Intracellular Pathogen , 2012, ACS chemical biology.

[4]  C. Bertozzi,et al.  Fluorogenic azidofluoresceins for biological imaging. , 2012, Journal of the American Chemical Society.

[5]  J. Eilers,et al.  Calcium rubies: a family of red-emitting functionalizable indicators suitable for two-photon Ca2+ imaging. , 2012, Journal of the American Chemical Society.

[6]  Michael Henze,et al.  Traceless tosylhydrazone-based triazole formation: a metal-free alternative to strain-promoted azide-alkyne cycloaddition. , 2012, Angewandte Chemie.

[7]  Christine Grienberger,et al.  Imaging Calcium in Neurons , 2012, Neuron.

[8]  S. Baylor,et al.  Calcium indicators and calcium signalling in skeletal muscle fibres during excitation-contraction coupling. , 2011, Progress in biophysics and molecular biology.

[9]  J. V. van Hest,et al.  Aza-dibenzocyclooctynes for fast and efficient enzyme PEGylation via copper-free (3+2) cycloaddition. , 2010, Chemical communications.

[10]  A. Salic,et al.  Metabolic labeling and direct imaging of choline phospholipids in vivo , 2009, Proceedings of the National Academy of Sciences.

[11]  Carolyn R Bertozzi,et al.  Bioorthogonal chemistry: fishing for selectivity in a sea of functionality. , 2009, Angewandte Chemie.

[12]  B. Sabatini,et al.  Calcium Signaling in Dendrites and Spines: Practical and Functional Considerations , 2008, Neuron.

[13]  Timothy J. Mitchison,et al.  A chemical method for fast and sensitive detection of DNA synthesis in vivo , 2008, Proceedings of the National Academy of Sciences.

[14]  Andrew L. Miller,et al.  Calcium transients and calcium signalling during early neurogenesis in the amphibian embryo Xenopus laevis. , 2006, Biochimica et biophysica acta.

[15]  S. Wray,et al.  Calcium signalling in smooth muscle. , 2005, Cell calcium.

[16]  A. Miyawaki,et al.  Circadian Dynamics of Cytosolic and Nuclear Ca2+ in Single Suprachiasmatic Nucleus Neurons , 2003, Neuron.

[17]  M. G. Finn,et al.  Click Chemistry: Diverse Chemical Function from a Few Good Reactions. , 2001, Angewandte Chemie.

[18]  M. Ohkura,et al.  A high signal-to-noise Ca2+ probe composed of a single green fluorescent protein , 2001, Nature Biotechnology.

[19]  R. Tsien,et al.  Fluorescent indicators for Ca2+based on green fluorescent proteins and calmodulin , 1997, Nature.

[20]  J. Baldwin,et al.  A Selective Cleavage of Aromatic Benzyl Ethers Located ortho to a Carbonyl Group by Magnesium Bromide. , 1997 .

[21]  S. Kume,et al.  Calcium waves along the cleavage furrows in cleavage-stage Xenopus embryos and its inhibition by heparin , 1996, The Journal of cell biology.

[22]  M. Konishi,et al.  Resting cytoplasmic free Ca2+ concentration in frog skeletal muscle measured with fura-2 conjugated to high molecular weight dextran , 1995, The Journal of general physiology.

[23]  M. Poenie,et al.  New fluorescent calcium indicators designed for cytosolic retention or measuring calcium near membranes. , 1995, Biophysical journal.

[24]  F. Di Virgilio,et al.  Inhibition of Fura-2 sequestration and secretion with organic anion transport blockers. , 1990, Cell calcium.

[25]  R. Tsien,et al.  Fluorescent indicators for cytosolic calcium based on rhodamine and fluorescein chromophores. , 1989, The Journal of biological chemistry.

[26]  F. Di Virgilio,et al.  Fura-2 secretion and sequestration in macrophages. A blocker of organic anion transport reveals that these processes occur via a membrane transport system for organic anions. , 1988, Journal of immunology.

[27]  M. Goligorsky,et al.  Alpha1‐adrenergic stimulation and cytoplasmic free calcium concentration in cultured renal proximal tubular cells: Evidence for compartmentalization of quin‐2 and fura‐2 , 1986, Journal of cellular physiology.

[28]  R. Tsien,et al.  A new generation of Ca2+ indicators with greatly improved fluorescence properties. , 1985, The Journal of biological chemistry.

[29]  R. Tsien.  A non-disruptive technique for loading calcium buffers and indicators into cells , 1981, Nature.