Sepsis Correlated with Increased Erythrocyte Na+ Content and Na+-K+ Pump Activity

The aims of the present study were twofold: (1) simultaneous determinations of Na+ transport parameters of erythrocytes from 40 healthy donors and 28 septic patients as assessed by a score of severity of sepsis (SSS), and (2) examination of the correlation between the SSS and specific Na+ transport abnormalities. Erythrocytes were obtained and loaded with different ionic compositions and cellular Na+ contents before determination of the near-maximal Na+ pump rate (Vmax), the physiological extrusion rate of Na+ (v) and the number of ouabain-binding sites (Bmax). In erythrocytes from septic patients, the cellular Na+ content was 28% higher (p < 0.001), with no differences in water content compared to erythrocytes from healthy donors. This elevated Na+ content was accompanied by significantly higher values for Vmax (43%), v (24%) and Bmax (48%) of the Na+ pump in septic erythrocytes. Moreover, significant positive correlations existed between Vmax and SSS (p = 0.028) and between cellular Na+ content and SSS (p = 0.005). These data suggest that during sepsis, membrane alterations occur and result in an increased cellular Na+ content. Active Na+ transport (Vmax and v) was significantly stimulated, possibly as a consequence of a secondary response to the elevated Na+ of cells. Both cellular Na+ and Vmax correlated well with the severity of sepsis, suggesting that these altered transport parameters may reflect the progress of sepsis.

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