Kinetochore alignment within the metaphase plate is regulated by centromere stiffness and microtubule depolymerases

An automated, quantitative 4D image analysis method is used to track kinetochore dynamics in metaphase cells.

[1]  M. Swann,et al.  Anaphase Movements in the Living Cell: A Study With Phase Contrast and Polarized Light on Chick Tissue Cultures , 1948 .

[2]  M. Lampson,et al.  Sensing Chromosome Bi-Orientation by Spatial Separation of Aurora B Kinase from Kinetochore Substrates , 2009, Science.

[3]  G Danuser,et al.  Yeast kinetochore microtubule dynamics analyzed by high-resolution three-dimensional microscopy. , 2005, Biophysical journal.

[4]  Jesse C. Gatlin,et al.  Condensin regulates the stiffness of vertebrate centromeres. , 2009, Molecular biology of the cell.

[5]  B. McEwen,et al.  CENP-E is essential for reliable bioriented spindle attachment, but chromosome alignment can be achieved via redundant mechanisms in mammalian cells. , 2001, Molecular biology of the cell.

[6]  Gaudenz Danuser,et al.  Computational processing and analysis of dynamic fluorescence image data. , 2008, Methods in cell biology.

[7]  I. Vernos,et al.  Xkid, a Chromokinesin Required for Chromosome Alignment on the Metaphase Plate , 2000, Cell.

[8]  Michael J. Emanuele,et al.  Multiple mechanisms of chromosome movement in vertebrate cells mediated through the Ndc80 complex and dynein/dynactin , 2007, Chromosoma.

[9]  Bruce F. McEwen,et al.  Chromosome Fragments Possessing Only One Kinetochore Can Congress to the Spindle Equator , 1997, The Journal of cell biology.

[10]  E. Salmon,et al.  Hec1 and nuf2 are core components of the kinetochore outer plate essential for organizing microtubule attachment sites. , 2004, Molecular biology of the cell.

[11]  E. Salmon,et al.  Oscillatory movements of monooriented chromosomes and their position relative to the spindle pole result from the ejection properties of the aster and half-spindle , 1986, The Journal of cell biology.

[12]  L. Cassimeris,et al.  TOGp, the human homolog of XMAP215/Dis1, is required for centrosome integrity, spindle pole organization, and bipolar spindle assembly. , 2004, Molecular biology of the cell.

[13]  J. Ellenberg,et al.  Condensin I Stabilizes Chromosomes Mechanically through a Dynamic Interaction in Live Cells , 2006, Current Biology.

[14]  J. Loncarek,et al.  The spindle assembly checkpoint is satisfied in the absence of interkinetochore tension during mitosis with unreplicated genomes , 2008, The Journal of cell biology.

[15]  E D Salmon,et al.  Directional instability of kinetochore motility during chromosome congression and segregation in mitotic newt lung cells: a push-pull mechanism , 1993, The Journal of cell biology.

[16]  Paul L. Rosin.  Unimodal thresholding , 2001, Pattern Recognit..

[17]  J. Shabanowitz,et al.  Aurora B Phosphorylates Centromeric MCAK and Regulates Its Localization and Microtubule Depolymerization Activity , 2004, Current Biology.

[18]  Jonathon Howard,et al.  Kinesin-8 Motors Act Cooperatively to Mediate Length-Dependent Microtubule Depolymerization , 2009, Cell.

[19]  J. McIntosh,et al.  Unstable kinetochore-microtubule capture and chromosomal instability following deletion of CENP-E. , 2002, Developmental cell.

[20]  E. Nigg,et al.  Role of Hec1 in Spindle Checkpoint Signaling and Kinetochore Recruitment of Mad1/Mad2 , 2002, Science.

[21]  Gaudenz Danuser,et al.  Linking data to models: data regression , 2006, Nature Reviews Molecular Cell Biology.

[22]  C. Sunkel,et al.  The Condensin I Subunit Barren/CAP-H Is Essential for the Structural Integrity of Centromeric Heterochromatin during Mitosis , 2005, Molecular and Cellular Biology.

[23]  Hongtao Yu,et al.  Human Bub1 protects centromeric sister-chromatid cohesion through Shugoshin during mitosis , 2004, Proceedings of the National Academy of Sciences of the United States of America.

[24]  Athanasios Papoulis,et al.  Probability, Random Variables and Stochastic Processes , 1965 .

[25]  J. Peters,et al.  Cohesin Cleavage by Separase Required for Anaphase and Cytokinesis in Human Cells , 2001, Science.

[26]  J. Peters,et al.  Regulation of Human Separase by Securin Binding and Autocleavage , 2002, Current Biology.

[27]  J. Swedlow,et al.  The making of the mitotic chromosome: modern insights into classical questions. , 2003, Molecular cell.

[28]  Kevin Ke,et al.  The Distribution of Polar Ejection Forces Determines the Amplitude of Chromosome Directional Instability , 2009, Current Biology.

[29]  J. Ellenberg,et al.  Live-Cell Imaging Reveals a Stable Cohesin-Chromatin Interaction after but Not before DNA Replication , 2006, Current Biology.

[30]  A. Mikhailov,et al.  DNA Damage during Mitosis in Human Cells Delays the Metaphase/Anaphase Transition via the Spindle-Assembly Checkpoint , 2002, Current Biology.

[31]  J. Swedlow,et al.  Aurora B regulates MCAK at the mitotic centromere. , 2004, Developmental cell.

[32]  Stefan Hümmer,et al.  The Human Kinesin Kif18A Is a Motile Microtubule Depolymerase Essential for Chromosome Congression , 2007, Current Biology.

[33]  Linda Wordeman,et al.  The kinesin-8 motor Kif18A suppresses kinetochore movements to control mitotic chromosome alignment. , 2008, Developmental cell.

[34]  T. Kapoor,et al.  Chromosomes Can Congress to the Metaphase Plate Before Biorientation , 2006, Science.

[35]  K. Jaqaman,et al.  Robust single particle tracking in live cell time-lapse sequences , 2008, Nature Methods.

[36]  J. Ellenberg,et al.  Distinct functions of condensin I and II in mitotic chromosome assembly , 2004, Journal of Cell Science.

[37]  Kim Nasmyth,et al.  Segregating Sister Genomes: The Molecular Biology of Chromosome Separation , 2002, Science.

[38]  G. von Dassow,et al.  MCAK facilitates chromosome movement by promoting kinetochore microtubule turnover , 2007, The Journal of cell biology.

[39]  M. Jordan,et al.  Mechanism of mitotic block and inhibition of cell proliferation by taxol at low concentrations. , 1993, Proceedings of the National Academy of Sciences of the United States of America.

[40]  P. Meraldi,et al.  Kinetochore-generated pushing forces separate centrosomes during bipolar spindle assembly , 2009, The Journal of cell biology.

[41]  O. Cuvier,et al.  Chromosome Condensation by a Human Condensin Complex inXenopus Egg Extracts* , 2001, The Journal of Biological Chemistry.

[42]  Viji M. Draviam,et al.  Timing and checkpoints in the regulation of mitotic progression. , 2004, Developmental cell.

[43]  A. Murray,et al.  Localization of Mad2 to Kinetochores Depends on Microtubule Attachment, Not Tension , 1998, The Journal of cell biology.

[44]  K. Shirahige,et al.  Displacement and re-accumulation of centromeric cohesin during transient pre-anaphase centromere splitting , 2007, Chromosoma.

[45]  P. Hergert,et al.  Depletion of centromeric MCAK leads to chromosome congression and segregation defects due to improper kinetochore attachments. , 2003, Molecular biology of the cell.

[46]  P. Sorger,et al.  A dual role for Bub1 in the spindle checkpoint and chromosome congression , 2005, The EMBO journal.

[47]  Kim Nasmyth,et al.  Cleavage of Cohesin by the CD Clan Protease Separin Triggers Anaphase in Yeast , 2000, Cell.

[48]  A. Murray,et al.  The Xenopus Chromokinesin Xkid Is Essential for Metaphase Chromosome Alignment and Must Be Degraded to Allow Anaphase Chromosome Movement , 2000, Cell.

[49]  Timothy J. Mitchison,et al.  Kin I Kinesins Are Microtubule-Destabilizing Enzymes , 1999, Cell.

[50]  S. Diez,et al.  The kinesin-related protein MCAK is a microtubule depolymerase that forms an ATP-hydrolyzing complex at microtubule ends. , 2003, Molecular cell.

[51]  K. Oegema,et al.  A new mechanism controlling kinetochore-microtubule interactions revealed by comparison of two dynein-targeting components: SPDL-1 and the Rod/Zwilch/Zw10 complex. , 2008, Genes & development.