Ethanolic Extract of Black Cumin Seed Reduced Radical Reactive from Dimethylbenzanthracene Compounds

Dimethylbenzantracene is a compound that is radical reactive, genotoxic, and immunotoxic. Black cumin seeds have antioxidative properties. The study aimed to determine the effect of the black cumin seed ethanolic extract as an antioxidant by observing the secretion activity of ROI and NO peritoneal macrophages in Sprague Dawley (SD) rats induced by DMBA. This controlled experiment used 30 male SD rats. The test animals were randomly divided into six groups. Group I was given standard food and drink. Group II was given DMBA. Groups III, IV, and V were given EEBCS doses of 5, 25, and 125 mg orally for seven weeks, respectively. Group VI was given Imboost. From week 3 to week seven, all test animals were induced 10x20 DMBA, 2x per week.At week 16, all test animals were sacrificed and isolated and cultured peritoneal macrophage cells. The activity of ROI secretion and NO macrophage secretion was tested. Data on ROI secretion and NO levels were tested for ANOVA, followed by LSD test with a confidence level of 95%. The results showed that DMBA induction increased ROI secretion by macrophages and decreased NO.EEBCS secretion decreased ROI secretion and grew NO. The DMBA group had the lowest NO levels. This study concluded that EEBCS at doses 5 and 125 reduced the flow of ROI peritoneal macrophages in SD rats induced by DMBA but reduced NO levels.