Bioaerosol characterization by surface-enhanced Raman spectroscopy ( SERS )

It is demonstrated that bioaerosols can be characterized by their surface-enhanced Raman spectra. Preliminary studies showed that Raman spectra of aqueous suspensions of Escherichia coli , Pseudomonas aeruginosa , nd Salmonella typhimuriumcan be obtained by mixing them with a nanocolloidal suspension of silver. Adsorption of the microorganisms on the silver quenches fluorescence and yields greatly enhanced spectra. To assist in the interpretation of the spectra we also obtained SERS spectra of N -acetyl glucosamine and the amino acids L-lysine, D-alanine andD-glutamic acid.After baseline studies identified the spectral region of interest and the concentrations of microorganisms and colloid particles needed to maximize the Raman signal, aerosolized microorganisms were impacted and transferred to a colloidal suspension of silver nanoparticles to obtain reproducible Raman spectra. 2004 Elsevier Ltd. All rights reserved.

[1]  R. Goodacre,et al.  Discrimination of bacteria using surface-enhanced Raman spectroscopy. , 2004, Analytical chemistry.

[2]  Y. Ozaki,et al.  Near-Infrared Excited Surface-Enhanced Raman Scattering of Biological Molecules on Gold Colloid I: Effects of pH of the Solutions of Amino Acids and of Their Polymerization , 1999 .

[3]  Gediminas Mainelis,et al.  Collection of Airborne Microorganisms by Electrostatic Precipitation , 1999 .

[4]  T. Reponen,et al.  Survival of Bacteria on Respirator Filters , 1999 .

[5]  V. V. Kurilenko,et al.  Evaluation of Phospholipid and Fatty Acid Compositions as Chemotaxonomic Markers of Alteromonas-Like Proteobacteria , 2000, Current Microbiology.

[6]  Paul R. Carey,et al.  Biochemical Applications of Raman and Resonance Raman Spectroscopies , 1982 .

[7]  S. S. Kim,et al.  Sampling E. coli and B. subtilis bacteria bioaerosols by a new type of impactor with a cooled impaction plate , 2003 .

[8]  T. Reponen,et al.  Effect of sampling time and air humidity on the bioefficiency of filter samplers for bioaerosol collection , 2001 .

[9]  S. C. Hill,et al.  Conditional-sampling spectrograph detection system for fluorescence measurements of individual airborne biological particles. , 1996, Applied optics.

[10]  T. Reponen,et al.  Collection of airborne microorganisms by a new electrostatic precipitator , 2002 .

[11]  Tiina Reponen,et al.  Survival of Airborne Microorganisms During Swirling Aerosol Collection , 2000 .

[12]  B. Lendl,et al.  Multidimensional information on the chemical composition of single bacterial cells by confocal Raman microspectroscopy. , 2000, Analytical chemistry.

[13]  T. Reponen,et al.  Design and Collection Efficiency of a New Electrostatic Precipitator for Bioaerosol Collection , 2002 .

[14]  Paul M Pellegrino,et al.  Near-Infrared Surface-Enhanced-Raman-Scattering-Mediated Detection of Single Optically Trapped Bacterial Spores , 2003, Applied spectroscopy.

[15]  S. C. Hill,et al.  Aerosol-fluorescence spectrum analyzer: real-time measurement of emission spectra of airborne biological particles. , 1995, Applied optics.

[16]  B. V. Bronk,et al.  Silver Colloids Impregnating or Coating Bacteria , 1998 .

[17]  M. Albrecht,et al.  Plasma resonance enhancement of Raman scattering by pyridine adsorbed on silver or gold sol particles of size comparable to the excitation wavelength , 1979 .

[18]  Effect of electrical charges and fields on injury and viability of airborne bacteria , 2002, Biotechnology and bioengineering.

[19]  W. Smith,et al.  Preparation of Stable, Reproducible Silver Colloids for Use as Surface-Enhanced Resonance Raman Scattering Substrates , 2002 .

[20]  K Willeke,et al.  Effect of impact stress on microbial recovery on an agar surface , 1995, Applied and environmental microbiology.

[21]  T. Reponen,et al.  Electrical charges on airborne microorganisms , 2001 .

[22]  M. Forsman,et al.  A comparative study of different methods to determine the total number and the survival ratio of bacteria in aerobiological samples , 1997 .

[23]  J. Ho,et al.  Design of an instrument for real-time detection of bioaerosols using simultaneous measurement of particle aerodynamic size and intrinsic fluorescence. , 1997, Journal of aerosol science.

[24]  T. Reponen,et al.  Performance of Air-O-Cell, Burkard, and Button Samplers for total enumeration of airborne spores. , 2000, AIHAJ : a journal for the science of occupational and environmental health and safety.

[25]  F. Parker.  Applications of Infrared, Raman, and Resonance Raman Spectroscopy in Biochemistry , 1983 .