Optimization of Polysaccharide Extraction and Its Composition in Pre-Germinated Brown Rice ( pre-GBR )

The polysaccharides from pre-germinated brown rice (pre-GBR) was studied by water extraction and alcohol precipitation procedure. Besides the changes of proximate compositions in pre-GBR, the monosaccharide composition of pre-GBR were analyzed with gas chromatography(GC). A set of optimized water soaking extraction parameters were formed by analyzing and optimizing several major influential factors :extraction temperature of 95 °C , extraction time of 4h, solid -liquid ration of 1:30 (w/v), and water extraction 4 times. Three groups were obtained from pre-GBR by hot water soaking extraction and alcohol precipitation: PGBRS-30, PGBRS-50 and PGBRS-80 group through different concentration of ethanol. PGBRS was composed of glucose, ribose, arabinose and mannose. The monosaccharide composition of PGBRS-30 and PGBRS-50 were similar with monosaccharide of xylose and the PGRS-80 group had less xylose.

[1]  Jiaqi Shi,et al.  The effect of electrolyzed water on decontamination, germination and γ-aminobutyric acid accumulation of brown rice , 2013 .

[2]  Qi Huang,et al.  Extraction of polysaccharides from herbal Scutellaria barbata D. Don (Ban-Zhi-Lian) and their antioxidant activity. , 2012, Carbohydrate polymers.

[3]  Jeong-Yong Cho,et al.  Quantitative analyses of individual γ-Oryzanol (Steryl Ferulates) in conventional and organic brown rice (Oryza sativa L.) , 2012 .

[4]  H. Qian,et al.  The impact of germination on the characteristics of brown rice flour and starch. , 2012, Journal of the science of food and agriculture.

[5]  Cai Yong-hong Exploration of Extraction and Isolation Method of Plant Polysaccharides , 2011 .

[6]  A. Moongngarm,et al.  Comparison of chemical compositions and bioactive compounds of germinated rough rice and brown rice , 2010 .

[7]  Li Ma,et al.  Protective effects of pre-germinated brown rice diet on low levels of Pb-induced learning and memory deficits in developing rat. , 2010, Chemico-biological interactions.

[8]  Yijun Fan,et al.  Composition analysis and antioxidant activity of polysaccharide from Dendrobium denneanum. , 2009, International journal of biological macromolecules.

[9]  Feng Chen,et al.  Structural characterisation of polysaccharides purified from longan (Dimocarpus longan Lour.) fruit pericarp , 2009 .

[10]  Juntao Tang,et al.  Effects of components in the culture solution on peptides accumulation during germination of brown rice , 2009 .

[11]  Z. Jing Study on Molecular Structure of Pumpkin Polysaccharide AP1 by Spectrospic and Chromatographic Analysis and Atomic Force Microscopy , 2009 .

[12]  J. Vázquez,et al.  Controlled germination to enhance the functional properties of rice , 2008 .

[13]  Mouming Zhao,et al.  Anti-diabetic effects of polysaccharides from Opuntia monacantha cladode in normal and streptozotocin-induced diabetic rats , 2008 .

[14]  K. Morikawa,et al.  Effects of pre-germinated brown rice on depression-like behavior in mice , 2007, Pharmacology Biochemistry and Behavior.

[15]  C. Icard-Vernière,et al.  Changes in nutrient composition, phytate and cyanide contents and α-amylase activity during cereal malting in small production units in Ouagadougou (Burkina Faso) , 2004 .

[16]  P. Åman,et al.  Determination of β-Glucan Molecular Weight Using SEC with Calcofluor Detection in Cereal Extracts , 2003 .

[17]  Eiko Arai,et al.  Distribution of amylose, nitrogen, and minerals in rice kernels with various characters. , 2002, Journal of agricultural and food chemistry.

[18]  J. Delcour,et al.  Structural characterisation of water-extractable and water-unextractable arabinoxylans in wheat bran. , 2002 .

[19]  T. Maekawa,et al.  Induction of homogeneous rooting control in liquid cultured brown rice using hypoxic conditions. , 2000 .

[20]  B. Cannon,et al.  Rare fatty acids in brown fat are substrates for thermogenesis during arousal from hibernation. , 1989, The American journal of physiology.

[21]  T. Price Seed sprout production for human consumption - A review , 1988 .