Antioxidative Effect and Melanogenesis of Nelumbo nucifera Stamen Extract on Cultured Human Skin Melanoma Cells Injured by Hydrogen Peroxide

Abstract - To examine the antioxidative effect and melanogenesis of Nelumbo nucifera stamen (NNS) extract on hydrogen peroxide H 2 O 2 induced cytotoxicity in cultured human skin melanoma cells (SK-MEL-3), cell adhesion activity (CAA), tyrosinase inhibitory activity and total amount of melanin synthesis were measured by colorimetric assay. In this study, H 2 O 2 significantly decreased CAA, and CAA 50 value of H 2 O 2 was determined at 30 uM. In the antioxidative effect, NNS extract increased cell adhesion activity which was decreased by H 2 O 2 induced cytotoxicity, and also, tyrosinase activity and total amount of melanin were decreased by NNS extract. These results suggested that H 2 O 2 was highly toxic on cultured human skin melanoma cells and NNS extract showed the antioxidative and inhibitory effect of melanogenesis by the increased CAA, and the decresed tyrosinase activity and total amount of melanin synthesis. Key words - Tyrosinase activity, Cell adhesion activity, Cytotoxicity, Colorimetric assay, Inhibitory effect, Plant extract

[1]  김민규,et al.  연잎 에탄올 추출물의 항산화 효과 , 2012 .

[2]  B. Rosner,et al.  A prospective study of dietary flavonoid intake and incidence of epithelial ovarian cancer , 2007, International journal of cancer.

[3]  C-J. S. Lin,et al.  Kaempferol induces apoptosis in human lung non-small carcinoma cells accompanied by an induction of antioxidant enzymes. , 2007, Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association.

[4]  H. Tajmir-Riahi,et al.  An Overview of DNA and RNA Bindings to Antioxidant Flavonoids , 2007, Cell Biochemistry and Biophysics.

[5]  I. A. Shehata,et al.  Flavonoids and biological activities of Jussiaea repens , 2007, Natural product research.

[6]  Q. Peng,et al.  Fructus corni enhances endothelial cell antioxidant defenses. , 1998, General pharmacology.

[7]  Goberdhan P Dimri,et al.  Replicative senescence, aging and growth-regulatory transcription factors. , 1996, Biological signals.

[8]  J. Richardson,et al.  Oxygen free radicals and brain dysfunction. , 1991, The International journal of neuroscience.

[9]  T. Kuroki,et al.  Regulation of melanin synthesis of B16 mouse melanoma cells by 1 alpha, 25-dihydroxyvitamin D3 and retinoic acid. , 1985, Cancer research.

[10]  E. Borenfreund,et al.  A simple quantitative procedure using monolayer cultures for cytotoxicity assays (HTD/NR-90) , 1985 .

[11]  T. Mosmann.  Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. , 1983, Journal of immunological methods.

[12]  이지연,et al.  Antioxidant Activity and Tyrosinase Inhibitory Activity of Elscholtziae Splendense , 2007 .

[13]  M. Kitagawa,et al.  Proliferative activity of primary cutaneous melanocytic tumours , 2005, Virchows Archiv A.

[14]  김종수,et al.  Antioxidative effects of traditional medicinal plants on lipid peroxidation , 2005 .

[15]  Xianglin Shi,et al.  Role of molecular oxygen in the generation of hydroxyl and superoxide anion radicals during enzymatic Cr(VI) reduction and its implication to Cr(VI)-induced carcinogenesis. , 2000, Journal of environmental pathology, toxicology and oncology : official organ of the International Society for Environmental Toxicology and Cancer.

[16]  B. Choi,et al.  Screening of the Tyrosinase Inhibitors from Marine Algae and Medicinal Plants , 1998 .

[17]  S. U. Kim,et al.  Methylmercury-induced neurotoxicity in cerebral neuron culture is blocked by antioxidants and NMDA receptor antagonists. , 1996, Neurotoxicology.

[18]  桑田 健.  Proliferative activity of primary cutaneous melanocytic tumors , 1995 .