Guidelines for cloning, expression, purification and functional characterization of primary HIV-1 envelope glycoproteins.

[1]  John P. Moore,et al.  HIV-1 Envelope Trimer Design and Immunization Strategies To Induce Broadly Neutralizing Antibodies. , 2016, Trends in immunology.

[2]  E. Kellenberger,et al.  A single-residue change in the HIV-1 V3 loop associated with maraviroc resistance impairs CCR5 binding affinity while increasing replicative capacity , 2015, Retrovirology.

[3]  O. Hartley,et al.  CCR5 susceptibility to ligand-mediated down-modulation differs between human T lymphocytes and myeloid cells , 2015, Journal of leukocyte biology.

[4]  L. White,et al.  Single genome amplification and standard bulk PCR yield HIV-1 envelope products with similar genotypic and phenotypic characteristics. , 2015, Journal of virological methods.

[5]  A. Trkola,et al.  Different Infectivity of HIV-1 Strains Is Linked to Number of Envelope Trimers Required for Entry , 2015, PLoS pathogens.

[6]  Peter D. Kwong,et al.  Conformational dynamics of single HIV-1 envelope trimers on the surface of native virions , 2014, Science.

[7]  Tongqing Zhou,et al.  Structure and immune recognition of trimeric prefusion HIV-1 Env , 2014, Nature.

[8]  J. Novak,et al.  HIV-1 Envelope Glycan Moieties Modulate HIV-1 Transmission , 2014, Journal of Virology.

[9]  Letizia Da Sacco,et al.  HIV-1 gp120 Activates the STAT3/Interleukin-6 Axis in Primary Human Monocyte-Derived Dendritic Cells , 2014, Journal of Virology.

[10]  S. Marullo,et al.  Targeting Spare CC Chemokine Receptor 5 (CCR5) as a Principle to Inhibit HIV-1 Entry* , 2014, The Journal of Biological Chemistry.

[11]  C. Cheng‐Mayer,et al.  Emergence of CD4 Independence Envelopes and Astrocyte Infection in R5 Simian-Human Immunodeficiency Virus Model of Encephalitis , 2014, Journal of Virology.

[12]  Gianguido C. Cianci,et al.  CCR5 Conformations Are Dynamic and Modulated by Localization, Trafficking and G Protein Association , 2014, PloS one.

[13]  L. Stamatatos,et al.  Faculty Opinions recommendation of Cryo-EM structure of a fully glycosylated soluble cleaved HIV-1 envelope trimer. , 2014 .

[14]  John P. Moore,et al.  Crystal Structure of a Soluble Cleaved HIV-1 Envelope Trimer , 2013, Science.

[15]  A. Bartesaghi,et al.  Pre-fusion structure of trimeric HIV-1 envelope glycoprotein determined by cryo-electron microscopy , 2013, Nature Structural &Molecular Biology.

[16]  J. Mascola,et al.  Broadly neutralizing antibodies and the search for an HIV-1 vaccine: the end of the beginning , 2013, Nature Reviews Immunology.

[17]  John P. Moore,et al.  Structural Characterization of Cleaved, Soluble HIV-1 Envelope Glycoprotein Trimers , 2013, Journal of Virology.

[18]  P. Colin,et al.  HIV-1 exploits CCR5 conformational heterogeneity to escape inhibition by chemokines , 2013, Proceedings of the National Academy of Sciences.

[19]  W. Goddard,et al.  Use of G-Protein-Coupled and -Uncoupled CCR5 Receptors by CCR5 Inhibitor-Resistant and -Sensitive Human Immunodeficiency Virus Type 1 Variants , 2013, Journal of Virology.

[20]  M. Churchill,et al.  Macrophage‐tropic HIV‐1 variants from brain demonstrate alterations in the way gp120 engages both CD4 and CCR5 , 2013, Journal of leukocyte biology.

[21]  Tao Pan,et al.  Environmental perturbations lift the degeneracy of the genetic code to regulate protein levels in bacteria , 2012, Proceedings of the National Academy of Sciences.

[22]  T. Soukka,et al.  Escherichia coli–expressed near full length HIV-1 envelope glycoprotein is a highly sensitive and specific diagnostic antigen , 2012, BMC Infectious Diseases.

[23]  E. Arts,et al.  HIV-1 Resistance to Maraviroc Conferred by a CD4 Binding Site Mutation in the Envelope Glycoprotein gp120 , 2012, Journal of Virology.

[24]  S. Zolla-Pazner,et al.  Efficiency of Bridging-Sheet Recruitment Explains HIV-1 R5 Envelope Glycoprotein Sensitivity to Soluble CD4 and Macrophage Tropism , 2012, Journal of Virology.

[25]  J. Bull,et al.  Slow fitness recovery in a codon-modified viral genome. , 2012, Molecular biology and evolution.

[26]  Guillermo Sapiro,et al.  Structural Mechanism of Trimeric HIV-1 Envelope Glycoprotein Activation , 2012, PLoS pathogens.

[27]  Gil Amitai,et al.  A vast collection of microbial genes that are toxic to bacteria. , 2012, Genome research.

[28]  Young Do Kwon,et al.  Unliganded HIV-1 gp120 core structures assume the CD4-bound conformation with regulation by quaternary interactions and variable loops , 2012, Proceedings of the National Academy of Sciences.

[29]  P. Simmonds,et al.  Independent evolution of macrophage-tropism and increased charge between HIV-1 R5 envelopes present in brain and immune tissue , 2012, Retrovirology.

[30]  F. Baleux,et al.  A synthetic heparan sulfate-mimetic peptide conjugated to a mini CD4 displays very high anti- HIV-1 activity independently of coreceptor usage. , 2012, Chemistry & biology.

[31]  John P. Moore,et al.  How can HIV-type-1-Env immunogenicity be improved to facilitate antibody-based vaccine development? , 2012, AIDS research and human retroviruses.

[32]  John P. Moore,et al.  Multiple CCR5 Conformations on the Cell Surface Are Used Differentially by Human Immunodeficiency Viruses Resistant or Sensitive to CCR5 Inhibitors , 2011, Journal of Virology.

[33]  M. Churchill,et al.  CD4-binding site alterations in CCR5-using HIV-1 envelopes influencing gp120-CD4 interactions and fusogenicity. , 2011, Virology.

[34]  Esther Kellenberger,et al.  New Insights into the Mechanisms whereby Low Molecular Weight CCR5 Ligands Inhibit HIV-1 Infection* , 2010, The Journal of Biological Chemistry.

[35]  M. Trevethick,et al.  Ligand binding assays at equilibrium: validation and interpretation , 2010, British journal of pharmacology.

[36]  M. Churchill,et al.  An altered and more efficient mechanism of CCR5 engagement contributes to macrophage tropism of CCR5-using HIV-1 envelopes. , 2010, Virology.

[37]  J. Coffin,et al.  Comparison of standard PCR/cloning to single genome sequencing for analysis of HIV-1 populations. , 2010, Journal of virological methods.

[38]  J. Binley,et al.  Role of Complex Carbohydrates in Human Immunodeficiency Virus Type 1 Infection and Resistance to Antibody Neutralization , 2010, Journal of Virology.

[39]  Young Do Kwon,et al.  Structure of HIV-1 gp120 with gp41-interactive region reveals layered envelope architecture and basis of conformational mobility , 2009, Proceedings of the National Academy of Sciences.

[40]  Yuntao Wu,et al.  Chemokine Coreceptor Signaling in HIV-1 Infection and Pathogenesis , 2009, PLoS pathogens.

[41]  R. Doms,et al.  Adaptive Mutations in a Human Immunodeficiency Virus Type 1 Envelope Protein with a Truncated V3 Loop Restore Function by Improving Interactions with CD4 , 2009, Journal of Virology.

[42]  E. Bunnik,et al.  Changing sensitivity to broadly neutralizing antibodies b12, 2G12, 2F5, and 4E10 of primary subtype B human immunodeficiency virus type 1 variants in the natural course of infection. , 2009, Virology.

[43]  N. Xia,et al.  Expression and purification of soluble HIV-1 envelope glycoprotein gp160 mutant from Saccharomyces cerevisiae. , 2009, Journal of bioscience and bioengineering.

[44]  B. Walker,et al.  Tracking the culprit: HIV-1 evolution and immune selection revealed by single-genome amplification , 2009, The Journal of experimental medicine.

[45]  A. Perelson,et al.  Genetic identity, biological phenotype, and evolutionary pathways of transmitted/founder viruses in acute and early HIV-1 infection , 2009, The Journal of experimental medicine.

[46]  R. Siliciano,et al.  Elite Suppressor–Derived HIV-1 Envelope Glycoproteins Exhibit Reduced Entry Efficiency and Kinetics , 2009, PLoS pathogens.

[47]  John P. Moore,et al.  Resistance to CCR5 inhibitors caused by sequence changes in the fusion peptide of HIV-1 gp41 , 2009, Proceedings of the National Academy of Sciences.

[48]  E. Freire,et al.  Binding thermodynamics of the N-terminal peptide of the CCR5 coreceptor to HIV-1 envelope glycoprotein gp120. , 2009, Biochemistry.

[49]  D. Burton,et al.  Determinants Flanking the CD4 Binding Loop Modulate Macrophage Tropism of Human Immunodeficiency Virus Type 1 R5 Envelopes , 2009, Journal of Virology.

[50]  C. Chougnet,et al.  Immune dysregulation in human immunodeficiency virus infection: know it, fix it, prevent it? , 2009, Journal of internal medicine.

[51]  A. Katz,et al.  High-affinity binding of southern African HIV type 1 subtype C envelope protein, gp120, to the CCR5 coreceptor. , 2008, AIDS research and human retroviruses.

[52]  Hui Li,et al.  Identification and characterization of transmitted and early founder virus envelopes in primary HIV-1 infection , 2008, Proceedings of the National Academy of Sciences.

[53]  Ronald G. Collman,et al.  Signaling Mechanism of HIV-1 gp120 and Virion-Induced IL-1β Release in Primary Human Macrophages1 , 2008, The Journal of Immunology.

[54]  E. Arts,et al.  Natural Variation in the V3 Crown of Human Immunodeficiency Virus Type 1 Affects Replicative Fitness and Entry Inhibitor Sensitivity , 2007, Journal of Virology.

[55]  L. Pease,et al.  Gene splicing and mutagenesis by PCR-driven overlap extension , 2007, Nature Protocols.

[56]  Steven Wolinsky,et al.  The HIV Env variant N283 enhances macrophage tropism and is associated with brain infection and dementia , 2006, Proceedings of the National Academy of Sciences.

[57]  B. Clotet,et al.  Purifying selection of CCR5-tropic human immunodeficiency virus type 1 variants in AIDS subjects that have developed syncytium-inducing, CXCR4-tropic viruses. , 2006, The Journal of general virology.

[58]  F. Bontems,et al.  Expression of highly toxic genes in E. coli: special strategies and genetic tools. , 2006, Current protein & peptide science.

[59]  J. Albert,et al.  Selection of human immunodeficiency virus type 1 R5 variants with augmented replicative capacity and reduced sensitivity to entry inhibitors during severe immunodeficiency. , 2005, The Journal of general virology.

[60]  V. Burnett,et al.  Novel single-round PCR and cloning of full-length envelope genes of HIV-1 may yield new insight into biomolecular antibacterial drug development. , 2005, Journal of virological methods.

[61]  M. Parmentier,et al.  Mutation of the DRY Motif Reveals Different Structural Requirements for the CC Chemokine Receptor 5-Mediated Signaling and Receptor Endocytosis , 2005, Molecular Pharmacology.

[62]  E. Arts,et al.  Differences in the Fitness of Two Diverse Wild-Type Human Immunodeficiency Virus Type 1 Isolates Are Related to the Efficiency of Cell Binding and Entry , 2005, Journal of Virology.

[63]  David G Myszka,et al.  Solubilization, stabilization, and purification of chemokine receptors using biosensor technology. , 2005, Analytical biochemistry.

[64]  D. Kabat,et al.  Kinetic Factors Control Efficiencies of Cell Entry, Efficacies of Entry Inhibitors, and Mechanisms of Adaptation of Human Immunodeficiency Virus , 2005, Journal of Virology.

[65]  S. Shorte,et al.  CXCR4-Tropic HIV-1 Envelope Glycoprotein Functions as a Viral Chemokine in Unstimulated Primary CD4+ T Lymphocytes1 , 2004, Journal of Immunology.

[66]  Robyn L Stanfield,et al.  Genetic and Phenotypic Analyses of Human Immunodeficiency Virus Type 1 Escape from a Small-Molecule CCR5 Inhibitor , 2004, Journal of Virology.

[67]  H. Schuitemaker,et al.  In Vivo Evolution of X4 Human Immunodeficiency Virus Type 1 Variants in the Natural Course of Infection Coincides with Decreasing Sensitivity to CXCR4 Antagonists , 2004, Journal of Virology.

[68]  H. Schuitemaker,et al.  Decreasing sensitivity to RANTES (regulated on activation, normally T cell-expressed and -secreted) neutralization of CC chemokine receptor 5-using, non-syncytium-inducing virus variants in the course of human immunodeficiency virus type 1 infection. , 2003, The Journal of infectious diseases.

[69]  Robert Blumenthal,et al.  Sensitivity of HIV-1 to entry inhibitors correlates with envelope/coreceptor affinity, receptor density, and fusion kinetics , 2002, Proceedings of the National Academy of Sciences of the United States of America.

[70]  Gabriela Canziani,et al.  Rapid Progression to Simian AIDS Can Be Accompanied by Selection of CD4-Independent gp120 Variants with Impaired Ability To Bind CD4 , 2002, Journal of Virology.

[71]  R. Doms,et al.  CD4-Independent Use of Rhesus CCR5 by Human Immunodeficiency Virus Type 2 Implicates an Electrostatic Interaction between the CCR5 N Terminus and the gp120 C4 Domain , 2001, Journal of Virology.

[72]  K. Lundstrom,et al.  Semliki Forest virus vectors: efficient vehicles for in vitro and in vivo gene delivery , 2001, FEBS Letters.

[73]  J. Sodroski,et al.  Antibody 17b binding at the coreceptor site weakens the kinetics of the interaction of envelope glycoprotein gp120 with CD4. , 2001, Biochemistry.

[74]  R. Altmeyer,et al.  Processing, Stability, and Receptor Binding Properties of Oligomeric Envelope Glycoprotein from a Primary HIV-1 Isolate* , 2000, The Journal of Biological Chemistry.

[75]  J. Ball,et al.  A polymerase chain reaction method for the amplification of full-length envelope genes of HIV-1 from DNA samples containing single molecules of HIV-1 provirus. , 2000, Journal of virological methods.

[76]  G. Nisticó,et al.  gp120 Induces Cell Death in Human Neuroblastoma Cells Through the CXCR4 and CCR5 Chemokine Receptors , 2000, Journal of neurochemistry.

[77]  R. Doms,et al.  Use of a gp120 Binding Assay To Dissect the Requirements and Kinetics of Human Immunodeficiency Virus Fusion Events , 1999, Journal of Virology.

[78]  Y. Soda,et al.  Changes in and discrepancies between cell tropisms and coreceptor uses of human immunodeficiency virus type 1 induced by single point mutations at the V3 tip of the env protein. , 1999, Virology.

[79]  J. Sodroski,et al.  Structure of an HIV gp120 envelope glycoprotein in complex with the CD4 receptor and a neutralizing human antibody , 1998, Nature.

[80]  J. Mills,et al.  Modified semliki forest virus expression vector that facilitates cloning. , 1997, BioTechniques.

[81]  R. Doms,et al.  CD4-Independent Infection by HIV-2 Is Mediated by Fusin/CXCR4 , 1996, Cell.

[82]  J. Carpten,et al.  Modulation of non-templated nucleotide addition by Taq DNA polymerase: primer modifications that facilitate genotyping. , 1996, BioTechniques.

[83]  S. Oka,et al.  A naturally occurring single basic amino acid substitution in the V3 region of the human immunodeficiency virus type 1 env protein alters the cellular host range and antigenic structure of the virus , 1994, Journal of virology.

[84]  A. Cann,et al.  A single amino acid substitution in the V1 loop of human immunodeficiency virus type 1 gp120 alters cellular tropism , 1993, Journal of virology.

[85]  J. Moore,et al.  Virions of primary human immunodeficiency virus type 1 isolates resistant to soluble CD4 (sCD4) neutralization differ in sCD4 binding and glycoprotein gp120 retention from sCD4-sensitive isolates , 1992, Journal of virology.

[86]  I. Chen,et al.  Envelope proteins from clinical isolates of human immunodeficiency virus type 1 that are refractory to neutralization by soluble CD4 possess high affinity for the CD4 receptor. , 1991, Proceedings of the National Academy of Sciences of the United States of America.

[87]  A. Fauci,et al.  Characterization of GP120 binding to CD4 and an assay that measures ability of sera to inhibit this binding. , 1988, Journal of immunology.

[88]  Y. Cheng,et al.  Relationship between the inhibition constant (K1) and the concentration of inhibitor which causes 50 per cent inhibition (I50) of an enzymatic reaction. , 1973, Biochemical pharmacology.

[89]  C. Flanagan.  Receptor conformation and constitutive activity in CCR5 chemokine receptor function and HIV infection. , 2014, Advances in pharmacology.

[90]  W. Brownlow,et al.  Ultrasensitive Analysis of Binding Affinity of HIV Receptor and Neutralizing Antibody Using Solution-Phase Electrochemiluminescence Assay. , 2013, Journal of electroanalytical chemistry.

[91]  R. Doms,et al.  Molecular mechanisms of HIV entry. , 2012, Advances in experimental medicine and biology.

[92]  B. Margolin,et al.  Genetic variability of the human immunodeficiency virus: statistical and biological issues. , 1994, Annual review of genetics.

[93]  D. Burton,et al.  Edinburgh Research Explorer Variation in HIV-I R5 macrophage-tropism correlates with sensitivity to reagents that block envelope: CD4 interactions but not with sensitivity to other entry inhibitors , 2022 .