Hydrolysis of phthalate esters by the gastro-intestinal contents of the rat.

The rates of hydrolysis in vitro of six phthalate diesters, namely dimethyl, diethyl, di-n-butyl, dicyclohexyl. di-n-octyl and di-(2-ethylhexyl) phthalate, were greatest in the presence of rat small-intestine contents and much slower with caecal or stomach contents. Dimethyl, diethyl and di-n-butyl phthalate were metabolized much more quickly than the rest. Enzymes of animal rather than bacterial origin appeared to be involved. The product of metabolism in every case was the corresponding monoester, the identity of the metabolite being established by thin-layer chromatography and mass and infra-red spectroscopy. There was a correlation between the rate of metabolism and the acute toxicity of the diesters in the rat.

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