Survey of Aedes triseriatus (Diptera: Culicidae) for Lacrosse Encephalitis Virus and West Nile Virus in Lorain County, Ohio

Abstract From June through September 2003, we conducted a survey of female Aedes triseriatus (Say) for infection with La Crosse encephalitis virus (family Bunyaviridae, genus Orthobunyavirus, LACV) and West Nile virus (family Flaviviridae, genus Flavivirus, WNV) at three locations in Lorain County, Ohio. To determine infection rate and seasonal variation of both viruses in the Ae. triseriatus population, Ae. triseriatus were collected weekly by using gravid traps and CO2-baited CDC light traps and tested for virus by using reverse transcriptase polymerase chain reaction. In total, 170 pools comprised of 2,143 females were tested for LACV, of which seven were positive; the maximum likelihood estimate of infection rate combined throughout the season was 3.22/1,000. None of 170 pools comprised of 2,158 females tested for WNV were positive. LACV-positive pools were detected between late July and early September.

[1]  Daniel R. O’Leary,et al.  Epidemiology and Transmission Dynamics of West Nile Virus Disease , 2005, Emerging infectious diseases.

[2]  P. Daszak,et al.  West Nile Virus Risk Assessment and the Bridge Vector Paradigm , 2005, Emerging infectious diseases.

[3]  Jamie A. Blow,et al.  An Update on the Potential of North American Mosquitoes (Diptera: Culicidae) to Transmit West Nile Virus , 2005, Journal of medical entomology.

[4]  M. Garvin,et al.  West Nile virus in mosquitoes of Northern Ohio, 2001-2002. , 2004, The American journal of tropical medicine and hygiene.

[5]  R. Lampman,et al.  Problems in Estimating Mosquito Infection Rates Using Minimum Infection Rate , 2003, Journal of medical entomology.

[6]  West Nile virus activity--United States, September 12-18, 2002, and Ohio, January 1-September 12, 2002. , 2002, MMWR. Morbidity and mortality weekly report.

[7]  M. Turell,et al.  Potential North American Vectors of West Nile Virus , 2001, Annals of the New York Academy of Sciences.

[8]  J. Roehrig,et al.  Widespread West Nile virus activity, eastern United States, 2000. , 2001, Emerging infectious diseases.

[9]  D. J. White,et al.  West Nile virus infection in birds and mosquitoes, New York State, 2000. , 2001, Emerging infectious diseases.

[10]  L. Kramer,et al.  West Nile virus infection in mosquitoes, birds, horses, and humans, Staten Island, New York, 2000. , 2001, Emerging infectious diseases.

[11]  J. Roehrig,et al.  Rapid Detection of West Nile Virus from Human Clinical Specimens, Field-Collected Mosquitoes, and Avian Samples by a TaqMan Reverse Transcriptase-PCR Assay , 2000, Journal of Clinical Microbiology.

[12]  C. G. Moore,et al.  La Crosse Encephalitis Virus Habitat Associations in Nicholas County, West Virginia , 2000, Journal of medical entomology.

[13]  D. Nash From the Centers for Disease Control and Prevention. Update: West Nile virus encephalitis--New York, 1999. , 1999, JAMA.

[14]  C. Apperson,et al.  Relative abundance and species composition of mosquito populations (Diptera:Culicidae) in a La Crosse virus-endemic area in western North Carolina. , 1996, Journal of medical entomology.

[15]  C. Apperson,et al.  Seasonal occurrence and abundance of Aedes triseriatus and other mosquitoes in a La Crosse virus-endemic area in western North Carolina. , 1996, Journal of the American Mosquito Control Association.

[16]  C. Blair,et al.  Reverse transcription-PCR detection of LaCrosse virus in mosquitoes and comparison with enzyme immunoassay and virus isolation , 1994, Journal of clinical microbiology.

[17]  C. G. Moore,et al.  GUIDELINES FOR ARBOVIRUS SURVEILLANCE PROGRAMS IN THE UNITED STATES , 1993 .

[18]  R. L. Berry,et al.  Aedes canadensis, a vector of La Crosse virus (California serogroup) in Ohio. , 1986, Journal of the American Mosquito Control Association.

[19]  R. Nasci Local variation in blood feeding by Aedes triseriatus and Aedes hendersoni (Diptera: Culicidae). , 1985, Journal of medical entomology.

[20]  G. Defoliart,et al.  Experimental La Crosse virus infection of red fox (Vulpes fulva), raccoon (Procyon lotor), opossum (Didelphis virginiana), and woodchuck (Marmota monax). , 1985, The American journal of tropical medicine and hygiene.

[21]  G. Clark,et al.  Occurrence of La Crosse (California serogroup) encephalitis viral infections in Illinois. , 1983, The American journal of tropical medicine and hygiene.

[22]  Burkot Tr,et al.  Bloodmeal sources of Aedes triseriatus and Aedes vexans in a southern Wisconsin forest endemic for La Crosse encephalitis virus. , 1982 .

[23]  Yuill Tm,et al.  Natural La Crosse virus infection in the red fox (Vulpes fulva), gray fox (urocyon cinereoargenteus), raccoon (Procyon lotor), and opossum (Didelphis virginiana). , 1981 .

[24]  T. Yuill,et al.  Natural La Crosse virus infection in the red fox (Vulpes fulva), gray fox (urocyon cinereoargenteus), raccoon (Procyon lotor), and opossum (Didelphis virginiana). , 1981, The American journal of tropical medicine and hygiene.

[25]  B. Miller,et al.  Vertical transmission of La Crosse virus (California encephalitis group): transovarial and filial infection rates in Aedes triseriatus (Diptera: Culicidae). , 1977, Journal of medical entomology.

[26]  B. LaLonde,et al.  Studies on the epidemiology of California encephalitis in an endemic area in Ohio in 1971. , 1975, The American journal of tropical medicine and hygiene.

[27]  W. H. Thompson,et al.  Observations on a natural cycle of La Crosse virus (California group) in Southwestern Wisconsin. , 1974, The American journal of tropical medicine and hygiene.

[28]  W. H. Thompson,et al.  Transovarial Transmission of LaCrosse Virus (California Encephalitis Group) in the Mosquito, Aedes triseriatus , 1973, Science.

[29]  R. Wright,et al.  Transmission of Lacrosse virus (California encephalitis group) by the mosquito Aedes triseriatus. , 1972, Journal of medical entomology.

[30]  W. H. Thompson,et al.  California group virus infections in small, forest-dwelling mammals of Wisconsin. Some ecological considerations. , 1971, The American journal of tropical medicine and hygiene.

[31]  Masterson Ra,et al.  California encephalitis--an endemic puzzle in Ohio. , 1971 .

[32]  H. Stegmiller,et al.  California encephalitis--an endemic puzzle in Ohio. , 1971, Health laboratory science.