Three‐dimensional automated nanoparticle tracking using Mie scattering in an optical microscope

The forward scattering of light in a conventional inverted optical microscope by nanoparticles ranging in diameter from 10 to 50 nm has been used to automatically and quantitatively identify and track their location in three‐dimensions with a temporal resolution of 200 ms. The standard deviation of the location of nominally stationary 50‐nm‐diameter nanoparticles was found to be about 50 nm along the light path and about 5 nm in the plane perpendicular to the light path. The method is based on oscillating the microscope objective along the light path using a piezo actuator and acquiring images with the condenser aperture closed to a minimum to enhance the effects of diffraction. Data processing in the time and spatial domains allowed the location of particles to be obtained automatically so that the technique has potential applications both in the processing of nanoparticles and in their use in a variety of fields including nanobiotechnology, pharmaceuticals and food processing where a simple optical microscope maybe preferred for a variety of reasons.

[1]  H. Masuhara,et al.  Spectral and 3-Dimensional Tracking of Single Gold Nanoparticles in Living Cells Studied by Rayleigh Light Scattering Microscopy† , 2009 .

[2]  Ben Ovryn,et al.  Three-dimensional forward scattering particle image velocimetry applied to a microscopic field-of-view , 2000 .

[3]  Ovryn,et al.  Imaging of transparent spheres through a planar interface using a high-numerical-aperture optical microscope , 2000, Journal of the Optical Society of America. A, Optics, image science, and vision.

[4]  Adam D. McFarland,et al.  Single Silver Nanoparticles as Real-Time Optical Sensors with Zeptomole Sensitivity , 2003 .

[5]  P. Doyle,et al.  Static and dynamic errors in particle tracking microrheology. , 2005, Biophysical journal.

[6]  S. Hell,et al.  Breaking the diffraction resolution limit by stimulated emission: stimulated-emission-depletion fluorescence microscopy. , 1994, Optics letters.

[7]  Charlie Gosse,et al.  Magnetic tweezers: micromanipulation and force measurement at the molecular level. , 2002, Biophysical journal.

[8]  Juan J de Pablo,et al.  Single nanoparticle tracking reveals influence of chemical functionality of nanoparticles on local ordering of liquid crystals and nanoparticle diffusion coefficients. , 2009, Nano letters.

[9]  Gang Wang.  Diffractive approach to image dynamic optically-bound assembly in real time. , 2006, Optics express.

[10]  Estimation of Centres and Radial Intensity Profiles of Spherical Nano‐Particles in Digital Microscopy , 2007, Biometrical journal. Biometrische Zeitschrift.

[11]  H. Balci,et al.  Three-dimensional particle tracking via bifocal imaging. , 2007, Nano letters.

[12]  T Wilson,et al.  Imaging of spheres with the confocal scanning optical microscope. , 1996, Optics letters.

[13]  Haw Yang,et al.  Rapid and Quantitative Sizing of Nanoparticles Using Three-Dimensional Single-Particle Tracking , 2007 .

[14]  3D particle positioning from CCD images using the generalized Lorenz–Mie and Huygens–Fresnel theories , 2006 .

[15]  G. A. Blab,et al.  Single nanoparticle photothermal tracking (SNaPT) of 5-nm gold beads in live cells. , 2006, Biophysical journal.

[16]  E. Patterson,et al.  Optical signatures of small nanoparticles in a conventional microscope. , 2008, Small.

[17]  E. Patterson,et al.  Tracking nanoparticles in an optical microscope using caustics. , 2008, Nanotechnology.

[18]  Viola Vogel,et al.  Interferometric optical detection and tracking of very small gold nanoparticles at a water-glass interface. , 2006, Optics express.