Detection of rpoB Mutations inMycobacterium tuberculosis by Biprobe Analysis

ABSTRACT A biprobe assay utilizing LightCycler technology was developed to detect rifampin resistance-associated gene mutations in theMycobacterium tuberculosis rpoB gene. Three biprobes detected all mutations present in the 46 rifampin-resistant isolates. Wild-type sequences were correctly identified in each case. The method was reproducible, accurate, and easy to use.

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