Blood flow velocity measurements in chicken embryo vascular network via PIV approach

A method for measuring of blood velocity in the native vasculature of a chick embryo by the method of micro anemometry from particle images (μPIV) is improved. A method for interrogation regions sorting by the mask of the vasculature is proposed. A method for sorting of the velocity field of capillary blood flow is implemented. The in vitro method was evaluated for accuracy in a glass phantom of a blood vessel with a diameter of 50 μm and in vivo on the bloodstream of a chicken embryo, by comparing the transverse profile of the blood velocity obtained by the PIV method with the theoretical Poiseuille laminar flow profile.

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