Characterization of zigzag patterns on the surface of bovine serum albumin films

Analysis of the patterns formed during the drying of biological fluids is employed for research and diagnosis in medicine and agriculture. Saline solutions of native proteins and DNA are characterized by zigzag patterns, which could be quantitatively described using the specific length of zigzags L specific . The aim of this study was to analyze a wide number of characteristics in order to identify those most sensitive to the effects of various physical and chemical factors on the protein structure. We examined the films prepared from salt solutions of bovine serum albumin (BSA) under varying conditions, viz.: a proportional change in the concentration of the components, substitution of NaCl with NaF and NaBr, temperature treatment, gamma irradiation, and addition of trivalent iron and biologically active flavin mononucleotide. The results revealed that the distribution of zigzag segment lengths was approximately lognormal, and the distribution of angles between the segments was approximately logistic. Two parameters appeared to be the most informative, viz. the average length of the segments, mainly depending on Cl – concentration, and the number of segments, depending on: Cl – concentration, concentration of the non-aggregated (but not necessarily unfragmented) protein, and the excess concentration of ions and low molecular weight compounds.

[1]  Wei Shen,et al.  Blood drop patterns: Formation and applications. , 2016, Advances in colloid and interface science.

[2]  N. Chebotareva,et al.  Kinetics of Thermal Denaturation and Aggregation of Bovine Serum Albumin , 2016, PloS one.

[3]  T. Yakhno.  Sodium chloride crystallization from drying drops of albumin–salt solutions with different albumin concentrations , 2015 .

[4]  A. Roshal,et al.  Textures on the surface of BSA films with different concentrations of sodium halides and water state in solution , 2015, Nanoscale Research Letters.

[5]  Josmary R. Silva,et al.  Morphological Analysis and Interaction of Chlorophyll and BSA , 2014, BioMed research international.

[6]  H. Ojha,et al.  Protective effect of ferulic acid on ionizing radiation induced damage in bovine serum albumin , 2014 .

[7]  T. Yakhno.  Complex Pattern Formation in Sessile Droplets of Protein-Salt Solutions with Low Protein Content. What Substance Fabricates These Patterns? , 2012 .

[8]  A. Mukherjee,et al.  Comparative study of flavins binding with human serum albumin: a fluorometric, thermodynamic, and molecular dynamics approach. , 2012, Chemphyschem : a European journal of chemical physics and physical chemistry.

[9]  A. Sarvazyan,et al.  Protein Self-Organization Patterns in Dried Serum Reveal Changes in B-Cell Disorders , 2012, Molecular Diagnosis & Therapy.

[10]  Stefano Benedettelli,et al.  Self-Organized Crystallization Patterns from Evaporating Droplets of Common Wheat Grain Leakages as a Potential Tool for Quality Analysis , 2011, TheScientificWorldJournal.

[11]  Wen Tang,et al.  Binding mechanism of halide ions to bovine serum albumin and hemoglobin: investigated by ion selective-electrode , 2011 .

[12]  Gauss M. Cordeiro,et al.  The generalized inverse Weibull distribution , 2011 .

[13]  M. Kassaee,et al.  Radiation synthesis and characterization of protein stabilized gold nanoparticles , 2010 .

[14]  Nicolaas Busscher,et al.  Standardization of the Biocrystallization Method for Carrot Samples , 2010 .

[15]  Yazhou Zhang,et al.  Flavin‐sensitized Photo‐oxidation of Lysozyme and Serum Albumin , 2009, Photochemistry and photobiology.

[16]  Nicolaas Busscher,et al.  First tests of standardized biocrystallization on milk and milk products , 2009 .

[17]  Feng Wang,et al.  Spectroscopic investigation of the interaction between riboflavin and bovine serum albumin , 2008 .

[18]  Maria Olga Kokornaczyk,et al.  Organic wheat quality from a defined Italian field-trial. , 2008 .

[19]  Xiaolong Xu,et al.  Oxygen-dependent Oxidation of Fe(II) to Fe(III) and Interaction of Fe(III) with Bovine Serum Albumin, Leading to a Hysteretic Effect on the Fluorescence of Bovine Serum Albumin , 2007, Journal of Fluorescence.

[20]  M. Gaber.  Effect of γ-irradiation on the molecular properties of bovine serum albumin , 2005 .

[21]  Michael Mitzenmacher,et al.  A Brief History of Generative Models for Power Law and Lognormal Distributions , 2004, Internet Math..

[22]  H. Haidara,et al.  Complex Pattern Formation in Drying Dispersions , 2002 .

[23]  C. S. Strom,et al.  Nucleation-limited aggregation of crystallites in fractal growth , 2000 .

[24]  Y. Cho,et al.  Effect of γ-Irradiation on the Molecular Properties of Bovine Serum Albumin and β-Lcatoglobulin , 2000 .

[25]  Allan Aasbjerg Nielsen,et al.  Computerised image analysis of biocrystallograms originating from agricultural products , 1999 .

[26]  E. Ben-Jacob,et al.  New periodic morphologies observed during dentritic growth of ammonium chloride crystals in thin layers , 1991 .

[27]  E. Ben-Jacob,et al.  The formation of patterns in non-equilibrium growth , 1990, Nature.

[28]  I. W. Burr.  Cumulative Frequency Functions , 1942 .