Comparative Evaluation of Antimicrobial Activity and Functional Properties of Native and Esterified Legume Proteins

Tow legume proteins (cowpea and common bean proteins) were isolated and esterified with methanol in the presence of hydrochloric acid for 10 h at 4°C to give esterification extent 82% and 79%; respectively. Esterification raised the pIs (iso-electric points) of legume proteins from pH 4 for the native legume proteins, to pH 6 in the case of cowpea protein and pH 8 in the case of common bean. Applying methylated proteins at four different concentrations (0.5, 0.75,1 and 2 mg/ml) to Petri dishes containing nutrient agar infected with two pathogenic Gramnegative (Enterobacter cloacae and Serratia marcescens) and Grampositive bacteria (Listeria monocytogenes) gave rise to concentrationdependent inhibition zones.

[1]  S. Chanda,et al.  Phytochemical screening and reported biological activities of some medicinal plants of Gujarat region , 2015 .

[2]  L. Coelho,et al.  Antimicrobial lectin from Schinus terebinthifolius leaf , 2013, Journal of applied microbiology.

[3]  M. Sitohy,et al.  Antimicrobial activity of native and esterified legume proteins against Gram-negative and Gram-positive bacteria , 2010 .

[4]  A. Rinaldi,et al.  Antimicrobial peptides: the LPS connection. , 2010, Methods in molecular biology.

[5]  Anima Nanda,et al.  Biosynthesis of silver nanoparticles from Staphylococcus aureus and its antimicrobial activity against MRSA and MRSE. , 2009, Nanomedicine : nanotechnology, biology, and medicine.

[6]  C. W. Coffmann,et al.  Functional properties and amino acid content of a protein isolate from mung bean flour , 2007 .

[7]  J. Takahashi,et al.  Antibacterial evaluation of 1,4-benzoquinone derivatives. , 2006, Journal of agricultural and food chemistry.

[8]  K. Lappalainen,et al.  Effect of temporin A modifications on its cytotoxicity and antimicrobial activity , 2005, APMIS : acta pathologica, microbiologica, et immunologica Scandinavica.

[9]  W. Michalski,et al.  Effect of chemical modification on anti-microbial and anti-viral properties of milk proteins , 2005 .

[10]  N. Narain,et al.  Functional properties of raw and heat processed cashew nut (Anacardium occidentale, L.) kernel protein isolates. , 2001, Die Nahrung.

[11]  M. Sitohy,et al.  Improvement of solubility and of emulsifying properties of milk proteins at acid pHs by esterification. , 2001, Die Nahrung.

[12]  M. Sitohy,et al.  STUDY OF FACTORS INFLUENCING PROTEIN ESTERIFICATION USING β‐LACTOGLOBULIN AS A MODEL , 2000 .

[13]  R. Hancock,et al.  Antibacterial Action of Structurally Diverse Cationic Peptides on Gram-Positive Bacteria , 2000, Antimicrobial Agents and Chemotherapy.

[14]  G. Doxastakis.  Lupin seed proteins , 2000 .

[15]  E. Carnovale,et al.  In-vitro Protein and Sulphur Amino Acid Availability as a Measure of Bean Protein Quality , 1992 .

[16]  J. Chobert,et al.  In vitro proteolysis and functional properties of reductively alkylated β-casein derivatives , 1991, Journal of Dairy Research.

[17]  T. Haertlé,et al.  Esterification of food proteins: characterization of the derivatives by a colorimetric method and by electrophoresis , 1991 .

[18]  J. Amiot,et al.  Functional and nutritional properties of acylated rapeseed proteins , 1990 .

[19]  J. Mossé.  Nitrogen-to-protein conversion factor for ten cereals and six legumes or oilseeds. A reappraisal of its definition and determination. Variation according to species and to seed protein content , 1990 .

[20]  T. Richardson,et al.  Selected functionality changes of beta-lactoglobulin upon esterification of side-chain carboxyl groups. , 1985, Journal of dairy science.

[21]  C. J. Brekke,et al.  Functional Properties of Acylated Pea Protein Isolates , 1983 .

[22]  K. Mcwatters,et al.  Whippability and Aeration , 1981 .

[23]  P. Halling.  Protein-stabilized foams and emulsions. , 1981, Critical reviews in food science and nutrition.

[24]  J. Duke.  Handbook of LEGUMES of World Economic Importance , 1982, Springer US.

[25]  A. Bauer,et al.  Antibiotic susceptibility testing by a standardized single disk method. , 1966, American journal of clinical pathology.