Passive defense in somatic embryos of Abies alba from two cell lines tested with Phaeolus schweinitzii.

Differential inhibition in growth of fungus Phaeolus schweinitzii inoculum was tested with somatic embryos of two cell lines of silver fir (Abies alba Mill.) by the method of dual cultures with pre-cultivation of somatic embryos and subsequent removal. It was confirmed that inhibitory substances from pre-cultivated somatic embryos L27 spreading to agar cultivation medium by simple diffusion cause greater inhibition of tester mycelial growth during subsequent co-cultivation compared to somatic embryos L122. Characteristics of embryogenic cell lines L27 and L122, from which somatic embryos were formed, are provided. Increased levels of peroxidase activity characterized the cell line L27. Natural non-induced constitutive defense of plant somatic embryos established by quantitatively measured restriction of growth in tester Phaeolus schweinitzii was discussed.

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