Effects of mechanical stimulation in osteogenic differentiation of bone marrow-derived mesenchymal stem cells on aligned nanofibrous scaffolds

Mechanical stimulation is one of the factors that regulating bone regeneration and healing. In this study, the biological responses of bone marrow derived mesenchymal stem cells (MSCs) to mechanical stimuli on aligned nanofibers and cast films were investigated. The uniaxial cyclic strain (1% strain and 1 Hz) was applied continuously to the cell substrates and osteoblastic activities were assessed at weeks 1, 2, and 4. The MSCs morphology on the aligned nanofibers was more elongated and spindle-like than MSCs on the cast films. Strain stimulation significantly attenuated the proliferation at week one but was significantly enhanced at week 4 for both types of substrates. Only the MSCs on strained nanofibers had greater alkaline phosphatase (ALP) levels at week one, while the ALP hindered the MSCs on both substrates at week 4. Strain application played a greater influence on osteocalcin expression for the cast films than the nanofibers at week 4. Clearly, the cellular response to strain induction was highly dependent on the surface—cell adhesion, which itself was greatly influenced by the surface texture of the substrate.

[1]  Zi Yin,et al.  The regulation of tendon stem cell differentiation by the alignment of nanofibers. , 2010, Biomaterials.

[2]  Yongnian Van,et al.  Controlled Adipose-derived Stromal Cells Differentiation into Adipose and Endothelial Cells in a 3D Structure Established by Cell-assembly Technique , 2009 .

[3]  L. Ghasemi‐Mobarakeh,et al.  Electrospun poly(epsilon-caprolactone)/gelatin nanofibrous scaffolds for nerve tissue engineering. , 2008, Biomaterials.

[4]  A. Papadimitropoulos,et al.  Effects of fluid flow and calcium phosphate coating on human bone marrow stromal cells cultured in a defined 2D model system. , 2008, Journal of biomedical materials research. Part A.

[5]  T. W. Pfeiler,et al.  Finite element modeling of 3D human mesenchymal stem cell-seeded collagen matrices exposed to tensile strain. , 2008, Journal of biomechanics.

[6]  Li-chi Han,et al.  Mechanical strain induces osteogenic differentiation: Cbfa1 and Ets-1 expression in stretched rat mesenchymal stem cells. , 2008, International journal of oral and maxillofacial surgery.

[7]  Laurence Vico,et al.  Ex Vivo bone formation in bovine trabecular bone cultured in a dynamic 3D bioreactor is enhanced by compressive mechanical strain. , 2008, Tissue engineering. Part A.

[8]  Ali Khademhosseini,et al.  Quantitative analysis of cell adhesion on aligned micro- and nanofibers. , 2008, Journal of biomedical materials research. Part A.

[9]  Patrick J. Prendergast,et al.  Regulatory Effects of Mechanical Strain on the Chondrogenic Differentiation of MSCs in a Collagen-GAG Scaffold: Experimental and Computational Analysis , 2008, Annals of Biomedical Engineering.

[10]  Benjamin Chu,et al.  Antithrombogenic property of bone marrow mesenchymal stem cells in nanofibrous vascular grafts , 2007, Proceedings of the National Academy of Sciences.

[11]  Elizabeth G Loboa,et al.  Osteogenic differentiation of human mesenchymal stem cells in collagen matrices: effect of uniaxial cyclic tensile strain on bone morphogenetic protein (BMP-2) mRNA expression. , 2006, Tissue engineering.

[12]  Seeram Ramakrishna,et al.  An aligned nanofibrous collagen scaffold by electrospinning and its effects on in vitro fibroblast culture. , 2006, Journal of biomedical materials research. Part A.

[13]  Song Li,et al.  Anisotropic mechanosensing by mesenchymal stem cells , 2006, Proceedings of the National Academy of Sciences.

[14]  Young-Mi Kang,et al.  Nanofiber alignment and direction of mechanical strain affect the ECM production of human ACL fibroblast. , 2005, Biomaterials.

[15]  Jennifer S. Park,et al.  Differential effects of equiaxial and uniaxial strain on mesenchymal stem cells , 2004, Biotechnology and bioengineering.

[16]  Yubo Sun,et al.  Effects of Cyclic Compressive Loading on Chondrogenesis of Rabbit Bone‐Marrow Derived Mesenchymal Stem Cells , 2004, Stem cells.

[17]  M. Kotaki,et al.  Aligned biodegradable nanofibrous structure: a potential scaffold for blood vessel engineering. , 2004, Biomaterials.

[18]  Peter X Ma,et al.  Nano-fibrous scaffolding architecture selectively enhances protein adsorption contributing to cell attachment. , 2003, Journal of biomedical materials research. Part A.

[19]  C. Simmons,et al.  Cyclic strain enhances matrix mineralization by adult human mesenchymal stem cells via the extracellular signal-regulated kinase (ERK1/2) signaling pathway. , 2003, Journal of biomechanics.

[20]  K. Jepsen,et al.  Cyclic hydrostatic pressure enhances the chondrogenic phenotype of human mesenchymal progenitor cells differentiated in vitro , 2003, Journal of orthopaedic research : official publication of the Orthopaedic Research Society.

[21]  Thomas J Webster,et al.  Enhanced functions of osteoblasts on nanometer diameter carbon fibers. , 2002, Biomaterials.

[22]  Lutz Claes,et al.  Proliferation of human-derived osteoblast-like cells depends on the cycle number and frequency of uniaxial strain. , 2002, Journal of biomechanics.

[23]  J. Iwamoto,et al.  Effect of decreased physical activity on bone mass in exercise-trained young rats , 2002, Journal of orthopaedic science : official journal of the Japanese Orthopaedic Association.

[24]  K. Hayakawa,et al.  Dynamic reorientation of cultured cells and stress fibers under mechanical stress from periodic stretching. , 2001, Experimental cell research.

[25]  R A Brand,et al.  Primary adult human bone cells do not respond to tissue (continuum) level strains , 2001, Journal of orthopaedic science : official journal of the Japanese Orthopaedic Association.

[26]  A. Goodship,et al.  Investigation of bone changes in microgravity during long and short duration space flight: comparison of techniques , 2000, European journal of clinical investigation.

[27]  S. Bloomfield,et al.  Changes in musculoskeletal structure and function with prolonged bed rest. , 1997, Medicine and science in sports and exercise.

[28]  J. Houde,et al.  Humeral bone density losses after shoulder surgery and immobilization. , 1996, Journal of shoulder and elbow surgery.

[29]  R. Brand,et al.  Human osteoblasts from younger normal and osteoporotic donors show differences in proliferation and TGF beta-release in response to cyclic strain. , 1995, Journal of biomechanics.

[30]  T. Ogihara,et al.  Alterations of bone mineral density of the femurs in hemiplegia , 1995, Calcified Tissue International.

[31]  C. Chestnut,et al.  Bone mass and exercise , 1993 .

[32]  D. Murray,et al.  The effect of strain on bone cell prostaglandin E2 release: A new experimental method , 1990, Calcified Tissue International.

[33]  P. Bianco,et al.  Human bone cell enzyme expression and cellular heterogeneity: Correlation of alkaline phosphatase enzyme activity with cell cycle , 1990, Journal of cellular physiology.

[34]  Zuisei Kanno,et al.  Effects of mechanical strain on proliferation and differentiation of bone marrow stromal cell line ST2 , 2004, Journal of Bone and Mineral Metabolism.

[35]  D Kaspar,et al.  Dynamic cell stretching increases human osteoblast proliferation and CICP synthesis but decreases osteocalcin synthesis and alkaline phosphatase activity. , 2000, Journal of biomechanics.

[36]  C. Chesnut.  Bone mass and exercise. , 1993, The American journal of medicine.

[37]  L E Lanyon,et al.  Static vs dynamic loads as an influence on bone remodelling. , 1984, Journal of biomechanics.

[38]  H. Uhthoff,et al.  Current concepts of internal fixation of fractures. , 1980, Canadian journal of surgery. Journal canadien de chirurgie.