Protective roles of nitric oxide on seed germination and seedling growth of rice (Oryza sativa L.) under cadmium stress.
Abstract:Nitric oxide (NO) is a bioactive molecule in plants which mediates a variety of physiological processes and responses to biotic and abiotic stresses including heavy metals. In the present study, the effects of exogenous NO donor sodium nitroprusside (SNP) on rice seed germination and seedlings growth were investigated under Cd stress and a possible mechanism was postulated. The results indicated that 100μM Cd significantly decreased rice seed germination index, vigor index, root and shoot lengths as well as fresh weight compared to control. Exogenous SNP dose-dependently attenuated the inhibition of rice seed germination and thereafter seedling growth caused by Cd. The promoting effect was most pronounced at 30μM SNP. Cd exposure caused oxidative stress by elevating hydrogen peroxide (H2O2) and malondialdehyde (MDA) contents in root and shoot of rice seedlings. 30μM SNP counteracted partly Cd toxicity by reducing the H2O2 and MDA contents of Cd-exposed seedlings. Meanwhile, application of SNP markedly stimulated the activities of superoxide dismutases (SOD), ascorbate peroxidases (APX), guaiacol peroxidase (POD) and catalases (CAT) compared with Cd treatment alone, thereby indicating the enhancement of the antioxidative capacity in the root and shoot under Cd stress. In addition, addition of 30μM SNP increased accumulation of proline in both root and shoot. The Cd accumulation in seedlings was significant reduced by SNP, implicating that the protective role of SNP was responsible for preventing Cd accumulation. However, the effects of SNP were reverted by addition of cPTIO, a NO scavenger, suggesting the protective roles of SNP might be related to the induction of NO. Furthermore, K3Fe(CN)6 and [Formula: see text] / [Formula: see text] had no similar roles as SNP. Based on these results, it can be concluded that SNP exerted an advantageous effect on alleviating the inhibitory effect of Cd on rice seed germination and seedling growth, which might interact with NO.
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[1] K. Hu,et al. Protective roles of nitric oxide on germination and antioxidant metabolism in wheat seeds under copper stress , 2007, Plant Growth Regulation.
[2] L. Lamattina,et al. Nitric oxide stimulates seed germination and de-etiolation, and inhibits hypocotyl elongation, three light-inducible responses in plants , 2000, Planta.
[3] W. Cao,et al. Exogenous nitric oxide improves seed germination in wheat against mitochondrial oxidative damage induced by high salinity , 2009 .
[4] K. Dietz,et al. The relationship between metal toxicity and cellular redox imbalance. , 2009, Trends in plant science.
[5] J. R. Magalhaes,et al. Nitric oxide reduces the stress effects of aluminum on the process of germination and early root growth of rice , 2010 .
[6] D. Jiang,et al. Characterization of Cadmium Uptake and Translocation in a Cadmium-Sensitive Mutant of Rice (Oryza sativa L. ssp. japonica) , 2009, Archives of environmental contamination and toxicology.
[7] F. Gaymard,et al. Nitric Oxide Contributes to Cadmium Toxicity in Arabidopsis by Promoting Cadmium Accumulation in Roots and by Up-Regulating Genes Related to Iron Uptake1[W] , 2009, Plant Physiology.
[8] R. Kohli,et al. Nitric oxide (as sodium nitroprusside) supplementation ameliorates Cd toxicity in hydroponically grown wheat roots , 2008 .
[9] Li Ping Zhang,et al. Copper-induced proline synthesis is associated with nitric oxide generation in Chlamydomonas reinhardtii. , 2008, Plant & cell physiology.
[10] M. Benavides,et al. Nitric oxide protects sunflower leaves against Cd-induced oxidative stress , 2005 .
[11] D. Klessig,et al. Nitric oxide: a new player in plant signalling and defence responses. , 2004, Current opinion in plant biology.
[12] C. Kao,et al. Cadmium toxicity is reduced by nitric oxide in rice leaves , 2004, Plant Growth Regulation.
[13] D. Jiang,et al. Salicylic acid alleviates the toxicity effect of cadmium on germination, seedling growth, and amylase activity of rice , 2010 .
[14] I. D. Teare,et al. Rapid determination of free proline for water-stress studies , 1973, Plant and Soil.
[15] A. Chaoui,et al. Differential sensitivity to cadmium in germinating seeds of three cultivars of faba bean (Vicia faba L.) , 2008, Acta Physiologiae Plantarum.
[16] W. Bramlage,et al. Modified thiobarbituric acid assay for measuring lipid oxidation in sugar-rich plant tissue extracts , 1992 .
[17] L. Tao,et al. Roles of nitric oxide in alleviating heavy metal toxicity in plants. , 2010, Archives of biochemistry and biophysics.
[18] T. Fujiwara,et al. Cadmium transport and tolerance in rice: perspectives for reducing grain cadmium accumulation , 2012, Rice.
[19] A. Chaoui,et al. [Biochemical changes associated with cadmium and copper stress in germinating pea seeds (Pisum sativum L.)]. , 2005, Comptes rendus biologies.
[20] Hikmet Geçkil,et al. Effects of Metals on Seed Germination, Root Elongation, and Coleoptile and Hypocotyl Growth in Triticum aestivum and Cucumis sativus , 2002, Archives of environmental contamination and toxicology.
[21] A. Chaoui,et al. Cadmium impairs mineral and carbohydrate mobilization during the germination of bean seeds. , 2010, Ecotoxicology and environmental safety.
[22] M. Zarrouk,et al. Antioxidant defense system in leaves of Indian mustard (Brassica juncea) and rape (Brassica napus) under cadmium stress , 2009, Acta Physiologiae Plantarum.
[23] L. Toppi,et al. Response to cadmium in higher plants , 1999 .
[24] Yasemin Ekmekçi,et al. Effects of cadmium on antioxidant enzyme and photosynthetic activities in leaves of two maize cultivars. , 2008, Journal of plant physiology.
[25] I. Shamsi,et al. Interactive effects of cadmium and aluminum on growth and antioxidative enzymes in soybean , 2008, Biologia Plantarum.
[26] A. Mihoub,et al. Changements biochimiques induits par le cadmium et le cuivre au cours de la germination des graines de petit pois (Pisum sativum L.) , 2005 .
[27] E. Gwóźdź,et al. Nitric oxide stimulates seed germination and counteracts the inhibitory effect of heavy metals and salinity on root growth of Lupinus luteus , 2003 .
[28] Russell L. Jones,et al. Nitric Oxide in Seed Dormancy and Germination , 2007 .
[29] M. Posmyk,et al. Antioxidant enzymes activity and phenolic compounds content in red cabbage seedlings exposed to copper stress. , 2009, Ecotoxicology and environmental safety.
[30] W. Horst,et al. Effect of aluminium on lipid peroxidation, superoxide dismutase, catalase, and peroxidase activities in root tips of soybean (Glycine max) , 1991 .
[31] K. Asada,et al. Hydrogen Peroxide is Scavenged by Ascorbate-specific Peroxidase in Spinach Chloroplasts , 1981 .