High-precision motion of magnetic microrobot with ultrasonic levitation for 3-D rotation of single oocyte

In this study, we propose an innovative driving method for a microrobot. By using acoustic levitation, the microrobot can be levitated from the glass substrate. We are able to achieve positioning accuracy of less than 1 μm, and the response speed and output force are also significantly improved. Silicon-based microrobots can be made into diverse shapes using deep reactive-ion etching (DRIE). Using custom-designed microrobots allows for the 3-D rotational control of a single bovine oocyte. Orientation with an accuracy of 1° and an average rotation velocity of 3 rad/s are achieved. This study contributes to the biotechnology. In the study of oocytes/embryos, manipulation is used for the enucleation, microinjection, and investigation of the characteristics of oocytes, such as the meiotic spindle and zona pellucida using PolScope. These studies and their clinical applications involve the three-dimensional (3-D) rotation of mammalian oocytes. The overall out-of-plane and in-plane rotations of the oocyte are demonstrated by using an acoustically levitated microrobot. In addition, by using this approach, it becomes much easier to manipulate the cell to investigate the characteristics of the single cell and analyze its mechanical properties.

[1]  Bradley J. Nelson,et al.  Biological Cell Injection Using an Autonomous MicroRobotic System , 2002, Int. J. Robotics Res..

[2]  A. Mizuno,et al.  Evaluation of cell characteristics by step-wise orientational rotation using optoelectrostatic micromanipulation , 1997 .

[3]  Fumihito Arai,et al.  Multi-beam bilateral teleoperation of holographic optical tweezers. , 2012, Optics express.

[4]  Fumihito Arai,et al.  On-demand Production of Emulsion Droplets Over a Wide Range of Sizes , 2010, Adv. Robotics.

[5]  Yu Sun,et al.  Three-Dimensional Rotation of Mouse Embryos , 2012, IEEE Transactions on Biomedical Engineering.

[6]  Yuxiang Liu,et al.  Investigation of inclined dual-fiber optical tweezers for 3D manipulation and force sensing. , 2009, Optics express.

[7]  Jake J. Abbott,et al.  How Should Microrobots Swim? , 2009 .

[8]  Fumihito Arai,et al.  On-chip enucleation of an oocyte by untethered microrobots , 2014 .

[9]  Robert E. Apfel,et al.  Acoustic radiation pressure produced by a beam of sound , 1981 .

[10]  H. Rubinsztein-Dunlop,et al.  Orientation of biological cells using plane-polarized Gaussian beam optical tweezers , 2003, physics/0308105.

[11]  H. Shiku,et al.  Three dimensional microelectrode array device integrating multi-channel microfluidics to realize manipulation and characterization of enzyme-immobilized polystyrene beads , 2009 .

[12]  Fumihito Arai,et al.  On-Chip Enucleation of Bovine Oocytes using Microrobot-Assisted Flow-Speed Control , 2013, Micromachines.

[13]  K. Yoshikawa,et al.  Tilt control in optical tweezers. , 2008, Journal of biomedical optics.

[14]  Metin Sitti,et al.  Independent control of multiple magnetic microrobots in three dimensions , 2013, Int. J. Robotics Res..

[15]  T. Peura,et al.  The effect of recipient oocyte volume on nuclear transfer in cattle , 1998, Molecular reproduction and development.

[16]  Fumihito Arai,et al.  High-Speed Magnetic Microrobot Actuation in a Microfluidic Chip by a Fine V-Groove Surface , 2013, IEEE Transactions on Robotics.

[17]  E. O. J. Salbu,et al.  Compressible Squeeze Films and Squeeze Bearings , 1964 .

[18]  F. Arai,et al.  Accurate dispensing system for single oocytes using air ejection. , 2013, Biomicrofluidics.

[19]  Hsi-Wen Tung,et al.  Generating mobile fluidic traps for selective three-dimensional transport of microobjects , 2014 .

[20]  John K. Gilbert,et al.  Models of magnetism , 1998 .

[21]  E. Wolf,et al.  Karyoplast‐cytoplast volume ratio in bovine nuclear transfer embryos: Effect on developmental potential , 1997, Molecular reproduction and development.

[22]  Yuan-Li Liang,et al.  Cell rotation using optoelectronic tweezers. , 2010, Biomicrofluidics.

[23]  F. Arai,et al.  Driving method of microtool by horizontally arranged permanent magnets for single cell manipulation , 2010 .

[24]  Martin Johnson.  Manipulating the mouse embryo: A laboratory manual: by B. Hogan, F. Constantini and E. Lacy, Cold Spring Harbor Laboratory, 1986. $60.00 (332 pages) ISBN 0 87969 175 1 , 1986 .

[25]  Young-Ho Kim,et al.  An integrated bio cell processor for single embryo cell manipulation , 2004, 2004 IEEE/RSJ International Conference on Intelligent Robots and Systems (IROS) (IEEE Cat. No.04CH37566).

[26]  Vijay Kumar,et al.  Automated biomanipulation of single cells using magnetic microrobots , 2013, Int. J. Robotics Res..

[27]  K. Matsuda,et al.  Theoretical and experimental examination of near-field acoustic levitation. , 2002, The Journal of the Acoustical Society of America.

[28]  H. Inaba,et al.  Optical trapping and rotational manipulation of microscopic particles and biological cells using higher-order mode Nd:YAG laser beams , 1991 .

[29]  Fumihito Arai,et al.  On-chip microrobot for investigating the response of aquatic microorganisms to mechanical stimulation. , 2013, Lab on a chip.

[30]  Sadayuki Ueha,et al.  Near‐field acoustic levitation of planar specimens using flexural vibration , 1996 .

[31]  Joel Voldman,et al.  Dielectrophoretic registration of living cells to a microelectrode array. , 2004, Biosensors & bioelectronics.

[32]  Bradley J. Nelson,et al.  Modeling and Control of Untethered Biomicrorobots in a Fluidic Environment Using Electromagnetic Fields , 2006, Int. J. Robotics Res..

[33]  Yong Zhang,et al.  Effects of the removal of cytoplasm on the development of early cloned bovine embryos. , 2011, Animal reproduction science.

[34]  Bruce Randall Donald,et al.  Planning and control for microassembly of structures composed of stress-engineered MEMS microrobots , 2013, Int. J. Robotics Res..

[35]  Metin Sitti,et al.  Two-Dimensional Autonomous Microparticle Manipulation Strategies for Magnetic Microrobots in Fluidic Environments , 2012, IEEE Transactions on Robotics.

[36]  Manabu Shimizu,et al.  In vitro and in vivo developmental potential of nuclear transfer embryos using bovine cumulus cells prepared in four different conditions. , 2003, Cloning and stem cells.

[37]  Andreas Ritter,et al.  Manipulating The Mouse Embryo A Laboratory Manual , 2016 .

[38]  Taisuke Masuda,et al.  On-chip magnetically actuated robot with ultrasonic vibration for single cell manipulations. , 2011, Lab on a chip.