Functional organization of rat olfactory bulb analysed by the 2‐deoxyglucose method

The spatial patterns of activity elicited in the rat olfactory bulb under different odor conditions have been analysed using the 2‐deoxyglucose (2DG) technique. Rats were injected with 14C‐2DG, exposed to controlled environments of amyl acetate, camphor, cage air, dimethyl disulfide, and pure air and autoradiographs prepared by the method of Sokoloff. Amyl acetate was associated with regions of glomerular layer densities in the anterolateral and mid‐ to posteromedial parts of the bulbar circumference, as previously reported. The extents of the densities increased with increasing concentration. Camphor odor was associated with regions of increased density in the anterodorsal and mid‐ to posteromedial parts of the bulb. Exposure to cage air produced scattered densities in the posteromedial and posterolateral bulb. Exposure to dimethyl disulfide gave variable results. Pure air was associated with a minimal number of small dense foci.

[1]  Adrian Ed,et al.  Sensory discrimination with some recent evidence from the olfactory organ. , 1950 .

[2]  W. E. Clark,et al.  THE PROJECTION OF THE OLFACTORY EPITHELIUM ON THE OLFACTORY BULB IN THE RABBIT , 1951, Journal of neurology, neurosurgery, and psychiatry.

[3]  V. Mountcastle.  Modality and topographic properties of single neurons of cat's somatic sensory cortex. , 1957, Journal of neurophysiology.

[4]  Electrophysiology of olfactory bulb. , 1958, Journal of neurophysiology.

[5]  D. Hubel,et al.  Receptive fields, binocular interaction and functional architecture in the cat's visual cortex , 1962, The Journal of physiology.

[6]  J Y Lettvin,et al.  Chemical transmission in the nose of the frog. , 1965, The Journal of physiology.

[7]  M. M. Mozell,et al.  Quantitative stimulation of frog olfactory receptors. , 1969, Journal of Neurophysiology.

[8]  M. M. Mozell,et al.  Evidence for a Chromatographic Model of Olfaction , 1970, The Journal of general physiology.

[9]  T. Powell,et al.  The neuropil of the periglomerular region of the olfactory bulb. , 1971, Journal of cell science.

[10]  D. F. Mathews,et al.  Response Patterns of Single Neurons in the Tortoise Olfactory Epithelium and Olfactory Bulb , 1972, The Journal of general physiology.

[11]  L. J. Land.  Localized projection of olfactory nerves to rabbit olfactory bulb. , 1973, Brain research.

[12]  K. Døving,et al.  Selective degeneration of neurons in the olfactory bulb following prolonged odour exposure. , 1973, Brain research.

[13]  T. Getchell.  Unitary Responses in Frog Olfactory Epithelium to Sterically Related Molecules at Low Concentrations , 1974, The Journal of general physiology.

[14]  J. Kauer,et al.  Responses of olfactory bulb neurones to odour stimulation of small nasal areas in the salamander , 1974, The Journal of physiology.

[15]  G. Shepherd,et al.  Autoradiographic analysis of olfactory receptor projections in the rabbit. , 1974, Brain research.

[16]  André Duchamp,et al.  QUALITATIVE AND QUANTITATIVE DISCRIMINATION IN THE FROG OLFACTORY RECEPTORS: ANALYSIS FROM ELECTROPHYSIOLOGICAL DATA , 1974, Annals of the New York Academy of Sciences.

[17]  M. Reivich,et al.  Mapping of functional neural pathways by autoradiographic survey of local metabolic rate with (14C)deoxyglucose. , 1975, Science.

[18]  John S. Kauer,et al.  Local sites of activity-related glucose metabolism in rat olfactory bulb during olfactory stimulation , 1975, Brain Research.

[19]  J. Powers,et al.  Vomeronasal organ: critical role in mediating sexual behavior of the male hamster. , 1975, Science.

[20]  D. G. Moulton,et al.  Spatial patterning of response to odors in the peripheral olfactory system. , 1976, Physiological reviews.

[21]  R. C. Collins,et al.  Metabolic anatomy of focal motor seizures. , 1976, Archives of neurology.

[22]  C. Pfaffmann,et al.  Dimethyl disulfide: an attractant pheromone in hamster vaginal secretion. , 1976, Science.

[23]  F. Sharp.  Activity-related increases of glucose utilization associated with reduced incorporation of glucose into its derivatives , 1976, Brain Research.

[24]  F R Sharp,et al.  Laminar analysis of 2-deoxyglucose uptake in olfactory bulb and olfactory cortex of rabbit and rat. , 1977, Journal of neurophysiology.

[25]  L. C. Skeen.  Odor-induced patterns of deoxyglucose consumption in the olfactory bulb of the tree shrew,Tupaia glis , 1977, Brain Research.

[26]  D. Hornung,et al.  Factors influencing the differential sorption of odorant molecules across the olfactory mucosa , 1977, The Journal of general physiology.

[27]  D. Hubel,et al.  Anatomical demonstration of orientation columns in macaque monkey , 1978, The Journal of comparative neurology.

[28]  G M Shepherd,et al.  Adaptive properties of olfactory receptors analysed with odour pulses of varying durations. , 1978, The Journal of physiology.

[29]  R. Eccles.  The domestic pig as an experimental animal for studies on the nasal cycle. , 1978, Acta oto-laryngologica.

[30]  G. Shepherd,et al.  Responses of olfactory receptor cells to step pulses of odour at different concentrations in the salamander. , 1978, The Journal of physiology.

[31]  W. Drongelen,et al.  Unitary recordings of near threshold responses of receptor cells in the olfactory mucosa of the frog. , 1978, The Journal of physiology.