Retinal optical coherence tomography at 1  μm with dynamic focus control and axial motion tracking

Abstract. High-resolution optical coherence tomography (OCT) retinal imaging is important to noninvasively visualize the various retinal structures to aid in better understanding of the pathogenesis of vision-robbing diseases. However, conventional OCT systems have a trade-off between lateral resolution and depth-of-focus. In this report, we present the development of a focus-stacking OCT system with automatic focus optimization for high-resolution, extended-focal-range clinical retinal imaging by incorporating a variable-focus liquid lens into the sample arm optics. Retinal layer tracking and selection was performed using a graphics processing unit accelerated processing platform for focus optimization, providing real-time layer-specific en face visualization. After optimization, multiple volumes focused at different depths were acquired, registered, and stitched together to yield a single, high-resolution focus-stacked dataset. Using this system, we show high-resolution images of the retina and optic nerve head, from which we extracted clinically relevant parameters such as the nerve fiber layer thickness and lamina cribrosa microarchitecture.

[1]  John S Werner,et al.  In vivo imaging of the photoreceptor mosaic in retinal dystrophies and correlations with visual function. , 2006, Investigative ophthalmology & visual science.

[2]  Shu Liu,et al.  Evaluation of retinal nerve fiber layer progression in glaucoma: a study on optical coherence tomography guided progression analysis. , 2010, Investigative ophthalmology & visual science.

[3]  Robert J Zawadzki,et al.  Adaptive optics-optical coherence tomography: optimizing visualization of microscopic retinal structures in three dimensions. , 2007, Journal of the Optical Society of America. A, Optics, image science, and vision.

[4]  Zhuolin Liu,et al.  Adaptive optics optical coherence tomography at 1 MHz. , 2014, Biomedical optics express.

[5]  Jon Holmes.  Theory and applications of multi-beam OCT , 2008, Canterbury Workshop and School in Optical Coherence Tomography and Adaptive Optics.

[6]  C K Hitzenberger,et al.  Dynamic focus in optical coherence tomography for retinal imaging. , 2006, Journal of biomedical optics.

[7]  Norberto López-Gil,et al.  Ocular wave-front aberration statistics in a normal young population , 2002, Vision Research.

[8]  Kazuhiro Sasaki,et al.  Extended depth of focus adaptive optics spectral domain optical coherence tomography , 2012, Biomedical optics express.

[9]  C. Curcio.  Photoreceptor topography in ageing and age-related maculopathy , 2001, Eye.

[10]  Alfredo Dubra,et al.  Adaptive optics imaging of healthy and abnormal regions of retinal nerve fiber bundles of patients with glaucoma. , 2015, Investigative ophthalmology & visual science.

[11]  Mirza Faisal Beg,et al.  Optic Nerve Head Registration Via Hemispherical Surface and Volume Registration , 2010, IEEE Transactions on Biomedical Engineering.

[12]  R. T. Hart,et al.  Deformation of the lamina cribrosa and anterior scleral canal wall in early experimental glaucoma. , 2003, Investigative ophthalmology & visual science.

[13]  P. Mackenzie,et al.  Optic nerve head and peripapillary morphometrics in myopic glaucoma. , 2014, Investigative ophthalmology & visual science.

[14]  Michelle Cua,et al.  Morphological phenotyping of mouse hearts using optical coherence tomography , 2014, Journal of biomedical optics.

[15]  Robert J Zawadzki,et al.  Three-dimensional anterior segment imaging in patients with type 1 Boston Keratoprosthesis with switchable full depth range swept source optical coherence tomography , 2013, Journal of biomedical optics.

[16]  Yifan Jian,et al.  Wavefront sensorless adaptive optics optical coherence tomography for in vivo retinal imaging in mice. , 2014, Biomedical optics express.

[17]  R. Leitgeb,et al.  Extended focus high-speed swept source OCT with self-reconstructive illumination. , 2011, Optics express.

[18]  R. Leitgeb,et al.  Extended focus depth for Fourier domain optical coherence microscopy. , 2006, Optics letters.

[19]  Mirza Faisal Beg,et al.  Exact Surface Registration of Retinal Surfaces From 3-D Optical Coherence Tomography Images , 2015, IEEE Transactions on Biomedical Engineering.

[20]  Hans Zappe,et al.  Elastomeric lenses with tunable astigmatism , 2013, Light: Science & Applications.

[21]  Michelle Cua,et al.  In vivo imaging of human photoreceptor mosaic with wavefront sensorless adaptive optics optical coherence tomography. , 2015, Biomedical optics express.

[22]  Martin F. Kraus,et al.  In vivo lamina cribrosa micro-architecture in healthy and glaucomatous eyes as assessed by optical coherence tomography. , 2013, Investigative ophthalmology & visual science.

[23]  Peripapillary retinal nerve fibre layer thickness measurement with SD-OCT in normal and glaucomatous eyes: distribution and correlation with age. , 2013, International journal of ophthalmology.

[24]  J. Duker,et al.  Ultrahigh speed 1050nm swept source/Fourier domain OCT retinal and anterior segment imaging at 100,000 to 400,000 axial scans per second. , 2010, Optics express.

[25]  Kevin Wong,et al.  Real-time acquisition and display of flow contrast using speckle variance optical coherence tomography in a graphics processing unit , 2014, Journal of biomedical optics.

[26]  Bo Wang,et al.  In vivo three-dimensional characterization of the healthy human lamina cribrosa with adaptive optics spectral-domain optical coherence tomography. , 2014, Investigative ophthalmology & visual science.

[27]  Harald Sattmann,et al.  In vivo investigation of human cone photoreceptors with SLO/OCT in combination with 3D motion correction on a cellular level. , 2010, Optics express.

[28]  P. Mackenzie,et al.  Comparison of the Clinical Disc Margin Seen in Stereo Disc Photographs With Neural Canal Opening Seen in Optical Coherence Tomography Images , 2014, Journal of glaucoma.

[29]  Yifan Jian,et al.  GPU accelerated OCT processing at megahertz axial scan rate and high resolution video rate volumetric rendering , 2013, Photonics West - Biomedical Optics.

[30]  R. Zawadzki,et al.  Wavefront correction and high-resolution in vivo OCT imaging with an objective integrated multi-actuator adaptive lens. , 2015, Optics express.

[31]  George Zouridakis,et al.  3D modeling to characterize lamina cribrosa surface and pore geometries using in vivo images from normal and glaucomatous eyes , 2013, Biomedical optics express.

[32]  Hans E. Grossniklaus,et al.  Morphometric changes of the choriocapillaris and the choroidal vasculature in eyes with advanced glaucomatous changes , 2002, Vision Research.

[33]  M. D. Roberts,et al.  Glaucomatous cupping of the lamina cribrosa: a review of the evidence for active progressive remodeling as a mechanism. , 2011, Experimental eye research.

[34]  Kevin Wong,et al.  Graphics processing unit accelerated optical coherence tomography processing at megahertz axial scan rate and high resolution video rate volumetric rendering , 2013, Journal of biomedical optics.

[35]  Maciej Wojtkowski,et al.  Microscopic OCT imaging with focus extension by ultrahigh-speed acousto-optic tunable lens and stroboscopic illumination. , 2014, Optics express.

[36]  Panomsak Meemon,et al.  Gabor-based fusion technique for Optical Coherence Microscopy. , 2010, Optics express.

[37]  Adeel Ahmad,et al.  Computational adaptive optics for broadband optical interferometric tomography of biological tissue , 2012, Proceedings of the National Academy of Sciences.

[38]  Nathan D. Shemonski,et al.  Computed optical interferometric tomography for high-speed volumetric cellular imaging. , 2014, Biomedical optics express.

[39]  Joseph A. Izatt,et al.  Efficient sweep buffering in swept source optical coherence tomography using a fast optical switch , 2012, Biomedical optics express.

[40]  Dongwon Lee,et al.  Confocal Adaptive Optics Imaging of Peripapillary Nerve Fiber Bundles: Implications for Glaucomatous Damage Seen on Circumpapillary OCT Scans. , 2015, Translational vision science & technology.

[41]  Gadi Wollstein,et al.  Imaging of the Lamina Cribrosa in Glaucoma: Perspectives of Pathogenesis and Clinical Applications , 2013, Current eye research.